[1]刘泳欣,张云峰.氩气通过Cav-1抑制小鼠脑梗死后急性期血脑屏障通透性增加[J].卒中与神经疾病杂志,2024,31(06):547-550556.[doi:10.3969/j.issn.1007-0478.2024.06.007]
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氩气通过Cav-1抑制小鼠脑梗死后急性期血脑屏障通透性增加()
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《卒中与神经疾病》杂志[ISSN:1007-0478/CN:42-1402/R]

卷:
第31卷
期数:
2024年06期
页码:
547-550556
栏目:
论著
出版日期:
2024-12-20

文章信息/Info

文章编号:
1007-0478(2024)06-0547-05
作者:
刘泳欣张云峰
226000 南通大学医学院[刘泳欣(南通大学附属医院卒中中心)]; 南通大学附属医院卒中中心[张云峰(通信作者)]
关键词:
脑缺血再灌注损伤 血脑屏障通透性 氩气 血管内皮细胞 跨细胞转运
分类号:
R743.33
DOI:
10.3969/j.issn.1007-0478.2024.06.007
文献标志码:
A
摘要:
目的 探究氩气(Argon,Ar)对小鼠脑缺血再灌注(Cerebral ischemia reperfusion,CIR)后急性期血脑屏障(Blood-brain barrier,BBB)通透性的影响及其机制。方法 27只雄性美国癌症研究所引进(Institute of cancer research,ICR)小鼠随机分为假手术组(Sham组)、短暂性大脑中动脉栓塞(Transient middle cerebral artery occlusion,tMCAO)模型组(tMCAO组)和氩气吸入组(tMCAO+iAr组),每组各9只; 比较各组小鼠再灌注6 h后伊文思蓝(Evans blue)渗漏及神经功能评分; 蛋白免疫印迹和免疫组化检测缺血侧脑组织闭锁连接蛋白1(Zonula occludens 1,ZO-1)、网格蛋白(Clathrin)和小窝蛋白1(Caveolin-1,Cav-1)的表达水平。结果 与Sham组比较,tMCAO组小鼠缺血侧脑组织Evans blue渗漏增加,神经功能缺损加重; 缺血侧脑组织Cav-1和Clathrin蛋白表达水平增高,而ZO-1蛋白表达水平无明显变化; CD31标记的血管内皮细胞Cav-1荧光强度增加,而ZO-1荧光强度无明显变化; 与tMCAO组比较,氩气吸入组小鼠脑组织Evans blue渗漏减少,神经功能缺损改善,缺血侧脑组织中Cav-1蛋白表达水平降低和缺血半暗区CD31阳性内皮细胞中Cav-1荧光强度减弱。结论 氩气可能通过抑制小鼠脑缺血再灌注后急性期内皮细胞Cav-1介导的跨细胞转运、减轻BBB通透性增加来发挥神经保护作用。

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:江苏省研究生科研创新项目(KYCX22_3372); 南通市社会民生面上项目(MS22022058)
更新日期/Last Update: 2024-12-20