[1]邢雅杰,时晓,刘丹丹.淫羊藿苷抑制CXCL1/CXCR2信号通路对癫痫模型小鼠神经元损伤的影响[J].卒中与神经疾病杂志,2025,32(02):115-121.[doi:10.3969/j.issn.1007-0478.2025.02.003]
　Xing Yajie*,Shi Xiao*,Liu Dandan..The effect of icariin on neuronal damage in epileptic mice by inhibiting the CXCL1/CXCR2 signaling pathway[J].Stroke and Nervous Diseases,2025,32(02):115-121.[doi:10.3969/j.issn.1007-0478.2025.02.003]
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淫羊藿苷抑制CXCL1/CXCR2信号通路对癫痫模型小鼠神经元损伤的影响()
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《卒中与神经疾病》杂志[ISSN:1007-0478/CN:42-1402/R]

卷:
第32卷
期数:
2025年02期
页码:
115-121
栏目:
癫痫
出版日期:
2025-04-20

文章信息/Info

Title:
The effect of icariin on neuronal damage in epileptic mice by inhibiting the CXCL1/CXCR2 signaling pathway
文章编号:
1007-0478(2025)02-0115-07
作者:
邢雅杰; 时晓; 刘丹丹
056002 河北省邯郸市第一医院儿科(邢雅杰 时晓); 邯郸市中心医院儿科(刘丹丹)
Author(s):
Xing Yajie*;  Shi Xiao*;  Liu Dandan.
*Department of Pediatrics,Handan First Hospital,Handan 056002
关键词:
癫痫 淫羊藿苷 C-X-C基序趋化因子1/C-X-C趋化因子受体2信号通路 神经元损伤
Keywords:
Epilepsy Icariin C-X-C motif chemokine 1/C-X-C chemokine receptor 2 signaling pathway Neuronal damage
分类号:
R742.1
DOI:
10.3969/j.issn.1007-0478.2025.02.003
文献标志码:
A
摘要:
目的 探究淫羊藿苷(Icariin,ICA)抑制C-X-C基序趋化因子1(C-X-C motif chemokine 1,CXCL1)/C-X-C趋化因子受体2(C-X-C chemokine receptor 2,CXCR2)信号通路对癫痫(Epilepsy,EP)模型小鼠神经元损伤的影响。方法 构建EP小鼠模型,将造模成功的EP模型小鼠随机分为EP组、淫羊藿苷低、中、高剂量组(ICA-L组、ICA-M组、ICA-H组)、淫羊藿苷高剂量+CXCL1重组蛋白组(ICA-H+CXCL1重组蛋白组),另取18只正常小鼠作对照组; 检测小鼠的行为学; 用改良神经功能缺损程度量表(Modified neuropathy symptom score,mNSS)评分评价小鼠神经功能缺损程度; 检测小鼠海马组织中炎症和氧化应激水平; 苏木精—伊红染色法(Hematoxylin-eosin staining,HE)染色观察小鼠海马组织病理形态; 原位末端转移酶标记技术(TdT-mediated dUTP Nick-End Labeling,TUNEL)检测小鼠神经元凋亡; 免疫印迹检测通路蛋白表达水平。结果 与Control组比较,EP组小鼠神经细胞出现损伤,结构不完整,细胞排列紊乱,数量减少,细胞皱缩,行为学评分、mNSS评分、白介素(Interleukin,IL)-1β、IL-6、肿瘤坏死因子(Tumor necrosis factor,TNF)-α、丙二醛(Malonaldehyde,MDA)水平、凋亡率升高,超氧化物歧化酶(Superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)水平下降,CXCL1,CXCR2表达水平上调(P<0.05); 与EP组比较,ICA-L,ICA-M,ICA-H组神经元损伤逐渐减轻,数量增多,细胞排列逐渐整齐,行为学评分、mNSS评分、IL-1β,IL-6,TNF-α,MDA水平、凋亡率降低,SOD,GSH-Px水平上升,CXCL1,CXCR2表达水平下调(P<0.05); 与ICA-H组比较,ICA-H+CXCL1重组蛋白组神经元损伤加重,行为学评分、mNSS评分、IL-1β,IL-6,TNF-α,MDA水平、凋亡率上升,SOD,GSH-Px水平下降,CXCL1,CXCR2表达水平上调(P<0.05)。结论 淫羊藿苷通过抑制CXCL1/CXCR2信号通路来抑制神经炎症和氧化应激,改善癫痫模型小鼠神经元损伤。
Abstract:
ObjectiveTo investigate the effect of icariin(ICA)on neuronal damage in epileptic(EP)mice by inhibiting the C-X-C motif chemokine 1(CXCL1)/C-X-C chemokine receptor 2(CXCR2)signaling pathway.Methods An EP mouse model was constructed, and successfully modeled EP mice were randomly assigned into EP group, icariin low, medium, and high dose groups(ICA-L group, ICA-M group, ICA-H group), icariin high dose+CXCL1 recombinant protein group(ICA-H+CXCL1 recombinant protein group), and 18 normal mice were selected as the control group. The behaviors of mice were tested. The Modified Neuropathy Symptom Score(mNSS)score was applied to evaluate the degree of neurological deficit in mice. The levels of inflammation and oxidative stress in mouse hippocampal tissue were detected. HE staining was applied to observe the pathological morphology of mouse hippocampal tissue. TUNEL was applied to detect neuronal apoptosis in mice. Immunoblotting was applied to detect the expression of pathway proteins.Results Compared with the Control group, the nerve cells in the EP group showed damage, incomplete structure, disordered cell arrangement, reduced number, and cell shrinkage. Besides, the behavioral scores, mNSS scores, IL-1β, IL-6, TNF-α, MDA levels, and apoptosis rate increased, the SOD and GSH-Px levels decreased, and the expression levels of CXCL1 and CXCR2 were upregulated(P<0.05). Compared with the EP group, the neuronal damage in the ICA-L, ICA-M, and ICA-H groups gradually decreased, the number increased, and the cell arrangement became more orderly. Additionally, the behavioral scores, mNSS scores, IL-1β, IL-6, TNF-α, MDA levels, and apoptosis rate decreased, the SOD and GSH-Px levels increased, and the expression level of CXCL1 and CXCR2 were downregulated(P<0.05). Compared with the ICA-H group, the ICA-H+CXCL1 recombinant protein group showed aggravated neuronal cell damage. Furthermore, the behavioral scores, mNSS scores, IL-1β, IL-6, TNF-α, MDA levels, and apoptosis rate increased, the SOD and GSH-Px levels decreased, and the expression level of CXCL1 and CXCR2 were upregulated(P<0.05).Conclusion Icariin suppresses neuroinflammation and oxidative stress, and improves neuronal damage in epileptic mice, by inhibiting the CXCL1/CXCR2 signaling pathway.

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更新日期/Last Update: 2025-04-20