[1]陈康 康玉琪 王艳等.APETX2对氯化锂-匹鲁卡品诱导痫性发作大鼠的影响及机制探究[J].卒中与神经疾病杂志,2016,23(01):30-35.[doi:10.3969/j.issn.1007-0478.2016.01.008]
 Chen Kang,Kang Yuqi,Wang Yan,et al.Effects of APETX2 on ethology of rat seizures induced by lithium chloride - pilocarpine induced and its mechanism exploration[J].Stroke and Nervous Diseases,2016,23(01):30-35.[doi:10.3969/j.issn.1007-0478.2016.01.008]
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APETX2对氯化锂-匹鲁卡品诱导痫性发作大鼠的影响及机制探究()
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《卒中与神经疾病》杂志[ISSN:1007-0478/CN:42-1402/R]

卷:
第23卷
期数:
2016年01期
页码:
30-35
栏目:
论著
出版日期:
2016-03-01

文章信息/Info

Title:
Effects of APETX2 on ethology of rat seizures induced by lithium chloride - pilocarpine induced and its mechanism exploration
作者:
陈康 康玉琪 王艳等
作者单位:430060 武汉,武汉大学人民医院神经内科[陈康 康玉琪 王艳 梁静静 卢祖能 肖哲曼(通信作者)]; 武汉大学基础学院病原生物学系(朱帆); 武汉大学基础医学院病理学与病理生理学系(何小华)
Author(s):
Chen Kang Kang Yuqi Wang Yanet al
Department of Neurology, Renmin Hospital of Wuhan University, Wuhan 430060
关键词:
APETx2 癫痫 行为学 钙成像 海马
Keywords:
APETX2 Epilepsy Ethology Calcium imaging Hippocampus
分类号:
R742.1
DOI:
10.3969/j.issn.1007-0478.2016.01.008
文献标志码:
A
摘要:
目的 探索APETX2对氯化锂-匹鲁卡品诱导痫性发作大鼠的行为学影响及可能的机制。方法 成年雄性SPF级SD大鼠18只,侧脑室置管后随机分为:癫痫组(9只)、APETx2组(9只),癫痫造模后观察2组癫痫大发作潜伏期及发作强度; APETx2处理原代培养海马神经元,动态观察其对钙成像的影响。结果 APETx2组的SD大鼠癫痫潜伏期延长,大发作程度减轻; APETx2处理原代培养海马神经元钙内流下降。结论 APETx2可抑制氯化锂-匹鲁卡品诱导SD大鼠痫性发作,减少酸诱导海马神经元钙离子浓度增加可能为机制之一。
Abstract:
ObjectiveTo study the effects of APETX2 on ethology of lithium chloride-pilocarpine induced seizures rat and possible mechanism.Methods Adult male Specific pathogen Free Sprague Dawley rats(n=18)were randomly divided into two groups after lateral ventricle intubating:APETX2 group(n=9)and control group(n=9). The latencies and severity of epileptic seizure were compared between two groupsafter building epileptic model. The effects of APETX2 on calcium imaging about primary hippocampal neurons were dynamically observed.Results APETX2 obviously prolonged the latencies of epileptic seizure and reduced seizure degree. Primary hippocampal neurons' calcium imaginginflowing decreased significantly in APETX2 group.Conclusion APETX2 can inhibit lithium chloride-pilocarpine induced rat seizures. One of its mechanisms may be that APETX2 reduced calcium ionconcentration enhancement induced by acid in hippocampal neurons.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(81471133); 湖北省自然科学基金(2014CFB734)
更新日期/Last Update: 1900-01-01