[1]曲友直,杨震,李敏,等.黄芪甲苷对脑缺血再灌注后血脑屏障的保护作用及IL-1β含量、MMP-9蛋白表达的影响[J].卒中与神经疾病杂志,2016,23(06):389-391.[doi:10.3969/j.issn.1007-0478.2016.06.001]
　Qu Youzhi,Yang Zhen,Li Min,et al.Protective effects of astragaloside IV on blood-brain barrier and the content of IL-1β and expression of MMP-9 protein after cerebral ischemic reperfusion[J].Stroke and Nervous Diseases,2016,23(06):389-391.[doi:10.3969/j.issn.1007-0478.2016.06.001]
点击复制

黄芪甲苷对脑缺血再灌注后血脑屏障的保护作用及IL-1β含量、MMP-9蛋白表达的影响()
分享到：

《卒中与神经疾病》杂志[ISSN:1007-0478/CN:42-1402/R]

卷:
第23卷
期数:
2016年06期
页码:
389-391
栏目:
论 著
出版日期:
2016-12-30

文章信息/Info

Title:
Protective effects of astragaloside IV on blood-brain barrier and the content of IL-1β and expression of MMP-9 protein after cerebral ischemic reperfusion
文章编号:
1007-0478(2016)06-0389-03
作者:
曲友直; 杨震; 李敏; 高国栋
710038 西安,第四军医大学唐都医院神经外科
Author(s):
Qu Youzhi; Yang Zhen; Li Min; et al.
Departerment of Neurosurgery,Tangdu Hospital 0f Fourth Military Medical University, Xi'an 710038
关键词:
黄芪甲苷 脑缺血再灌注 血脑屏障 IL-1β MMP-9
Keywords:
Astragaloside Ⅳ Cerebral ischemic reperfusion Blood-brain barrier IL-1β MMP-9
分类号:
R743
DOI:
10.3969/j.issn.1007-0478.2016.06.001
文献标志码:
A
摘要:
目的 探讨黄芪甲苷对大鼠脑缺血再灌注后血脑屏障的保护作用及其机制。方法 将SD大鼠72只随机等分为4组:假手术组、生理盐水对照组、小剂量黄芪甲苷治疗组(10 mg/kg)和大剂量黄芪甲苷治疗组(20 mg/kg),采用分光光度计法、酶联免疫吸附法及免疫组化法分别检测各组大鼠脑组织伊文氏蓝、IL-1β含量及MMP-9蛋白的表达水平。结果 与假手术组相比,生理盐水对照组脑组织伊文氏蓝含量明显增多、IL-1β含量显著增高、MMP-9蛋白的表达明显增强(P<0.01); 与生理盐水对照组相比,小剂量黄芪甲苷治疗组及大剂量黄芪甲苷治疗组脑组织伊文氏蓝含量均显著减少、IL-1β含量明显降低、MMP-9蛋白表达明显减弱(P<0.05); 小剂量黄芪甲苷治疗组与大剂量黄芪甲苷治疗组相比,伊文氏蓝、IL-1β含量及MMP-9蛋白表达无显著差异(P>0.05)。结论 黄芪甲苷对脑缺血再灌注后血脑屏障具有保护作用,这可能与其下调IL-1β含量、抑制MMP-9蛋白的表达有关。
Abstract:
ObjectiveTo investigate the protective effects of astragaloside IV on blood-brain barrier and the content of IL-1β, expression of MMP-9 protein after cerebral ischemic reperfusion.Methods 72 SD rats were divided randomly into four groups: sham-operated group, normal- saline contral group, group treated by low dose of astragaloside IV(10 mg/kg),group treated by high dose of astragaloside IV(20 mg/kg).Evans Blue(EB)leakage, the content of IL-1β,expression of MMP-9 protein were respectively detected by spectrophotometry, ELISA and immunohistochemistry.Results Compared with sham-operated group, EB leakage,the content of IL-1β and expression of MMP-9 protein were higher in normal- saline contral group(P<0.01).Compared with normal saline contral group, EB leakage,the content of IL-1β and expression of MMP-9 protein were lower in group treated by low dose of astragaloside Ⅳ and group treated by high dose of astragaloside Ⅳ(P<0.05). There was no statistical difference between group treated by low dose of astragaloside Ⅳ and group treated by high dose of astragaloside Ⅳ(P>0.05).Conclusion Astragaloside Ⅳ could have protective effects on blood-brain barrier after cerebral ischemic reperfusion. Its mechanisms may be associated with down-regulation of the content of IL-1β and expression of MMP-9 protein.

参考文献/References:

[1] Nordal RA,Wong CS.Molecular targets in radiation-induced blood-brain barrier disruption[J].Int J Radiat Oncol Biol Phys,2005,62(1):279-287.
[2] Begley DJ,Brightman MW.Structural and functional aspects of the blood-brain barrier[J].Prog Drug Res,2003,61(1):39-78.
[3] Asahi M,Wang X,Mori T,et al.Effects of matrix metalloproteinase-9 gene knock-out on the proteolysis of blood-brain barrier and white matter components after cerebral ischemia[J].J Neurosci,2001,21(19):7724-7732.
[4] 胡芳弟,封士兰,赵健雄,等.HPLC法测定黄芪中黄酮类成分和黄芪甲苷的含量[J].分析测试技术与仪器,2003,9(3):173-177.
[5] 黄惠,王岭,李艳,等.黄芪注射液对大鼠脑缺血再灌注损伤的保护作用研究[J].卒中与神经疾病,2014,21(1):19-22.
[6] 曲友直,赵燕玲,李敏,等.黄芪甲苷对脑缺血再灌注后血脑屏障的保护作用及occludin蛋白表达的影响[J].卒中与神经疾病,2010,17(2):92-93, 96.
[7] Longa EZ,Weinstein PR,Carlson S,et al.Reversible middle cerebral artery occlusion without craniectomy in rats[J].Stroke,1989,20(1):84-91.
[8] 陈新云.炎症因子在大鼠脑缺血后MMP和TIMP-1致脑损伤中的作用[J].中山大学学报(医学科学版),2013,34(2):230-234.
[9] Gu Z,Cui J,Brown S,et al.A highly specific inhibitor of matrix metalloproteinase-9 rescues laminin from proteolysis and neurons from apoptosis in transient focal cerebral ischemia[J].J Neurosci,2005,25(27):6401-6408.
[10] Li M,Qu YZ,Zhao ZW,et al.Astragaloside IV protects against focal cerebral ischemia/reperfusion injury correlating to suppression of neutrophils adhesion-related molecules[J].Neurochem Int,2012,60(5):458-465.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金(30600841)
更新日期/Last Update: 2016-12-20