[1]李丹,卞冰阳,吴丹,等.一种观测脑疾病状态下胶质细胞活动的钙成像技术[J].卒中与神经疾病杂志,2017,24(04):286-289.[doi:10.3969/j.issn.1007-0478.2017.04.002]
 Li Dan,Bian Bingyang,Wu Dan,et al.Using calcium imaging to record glia activity in brain diseases[J].Stroke and Nervous Diseases,2017,24(04):286-289.[doi:10.3969/j.issn.1007-0478.2017.04.002]
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一种观测脑疾病状态下胶质细胞活动的钙成像技术()
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《卒中与神经疾病》杂志[ISSN:1007-0478/CN:42-1402/R]

卷:
第24卷
期数:
2017年04期
页码:
286-289
栏目:
论 著
出版日期:
2017-08-26

文章信息/Info

Title:
Using calcium imaging to record glia activity in brain diseases
文章编号:
1007-0478(2017)04-0282-04
作者:
李丹卞冰阳吴丹马洪涛张秀梅
130000 长春,吉林大学第一医院放射科[李丹 卞冰阳 吴丹 张秀梅(通信作者)]; 美国康奈尔大学医学院神经外科(马洪涛)
Author(s):
Li DanBian BingyangWu Danet al.
Department of Radiology,The First Hospital of Jilin University,Changchun 130000
关键词:
钙敏感性染料 广视野钙成像 胶质细胞 神经元 癫痫
Keywords:
Calcium sensitive dye Wide-field calcium imaging Glia Neuron Seizure
分类号:
R742
DOI:
10.3969/j.issn.1007-0478.2017.04.002
摘要:
目的 探讨应用广视野钙成像技术观测神经元和胶质细胞的活动。方法 采用对流强化法对麻醉雄性大鼠的新皮层进行钙染料染色,应用广视野钙成像技术观测药物诱导的急性癫痫模型中神经元和胶质细胞的活动。结果 钙成像的光学脑电图显示癫痫时的神经活动为快速传播的波,其传播范围仅限于致痫灶处,而胶质细胞活动由癫痫起源触发,但是表现为慢速传播的定型波,其传播范围超出神经活动范围。结论 广视野钙成像技术更有效地观测神经元和胶质细胞的活动并区别神经元和胶质细胞的网络活动。
Abstract:
ObjectiveTo record the neuronal and glial activity with wide-field calcium imaging.Methods Using convection-enhanced delivery based staining method, the rat neocortex was stained with calcium dye in vivo. The neuronal and glial activity in an acute pharmacologic model of neocortical seizures was imaged.Results Optical calcium-based ECoG maps revealed that neuronal waves propagated rapidly and remained mostly confined to the seizure focus. Glial waves were triggered by ictal onset but propagated slowly in a stereotypical fashion far beyond the seizure focus.Conclusion Wide-field calcium imaging was more effective in observing the activity of neurons and glias and distinguishing between neurons and glial cells from the network activities.

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备注/Memo

备注/Memo:
基金项目:吉林省科技发展计划项目(项目编号为20160414006GH); 吉林省科技发展计划项目(项目编号为20110462)
更新日期/Last Update: 2017-08-20