[1]吉文玉,蔡宁,杨思培.微小核糖核酸592对神经胶质瘤细胞株U251细胞凋亡影响的机制[J].卒中与神经疾病杂志,2017,24(04):319-322+341.[doi:10.3969/j.issn.1007-0478.2017.04.010]
　Ji Wenyu,Cai Ning,Yang Sipei..The miR-592 suppresses U251 cell apoptosis by targeting Runx2[J].Stroke and Nervous Diseases,2017,24(04):319-322+341.[doi:10.3969/j.issn.1007-0478.2017.04.010]
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微小核糖核酸592对神经胶质瘤细胞株U251细胞凋亡影响的机制()
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《卒中与神经疾病》杂志[ISSN:1007-0478/CN:42-1402/R]

卷:
第24卷
期数:
2017年04期
页码:
319-322+341
栏目:
论 著
出版日期:
2017-08-26

文章信息/Info

Title:
The miR-592 suppresses U251 cell apoptosis by targeting Runx2
文章编号:
1007-0478(2017)04-0319-05
作者:
吉文玉; 蔡宁; 杨思培
830054 乌鲁木齐,新疆医科大学第一附属医院儿外一科(吉文玉); 新疆维吾尔自治区中医医院神经外科(蔡宁); 苏州医科大学附属第二医院神经内科(杨思培)
Author(s):
Ji Wenyu;  Cai Ning;  Yang Sipei.
Deapartment of First Pediatric Surgery,The First Affiliated Hospital of Xinjiang Medical University, Wulumuqi 830054
关键词:
神经胶质瘤 U251 miR-592 凋亡 Runx2
Keywords:
Glioma MicroRNA miR-592 U251 Apoptotic rate
分类号:
R739.41
DOI:
10.3969/j.issn.1007-0478.2017.04.010
摘要:
目的 探讨微小核糖核酸592(miR-592)对神经胶质瘤细胞株U251凋亡的影响。方法 首先通过定量聚合酶链反应(PCR)分析miR-592在28份神经胶质瘤与其临近癌旁组织中的表达水平; 随后向U251细胞转染miR-592的拟合物,并通过流式细胞技术分析miR-592过表达对U251细胞凋亡的影响; 通过生物信息学分析找到miR-592的潜在靶分子,并通过荧光素酶双报告实验以及蛋白免疫印迹法等进行验证; 进一步转染U251细胞Runx2的下调siRNA,绘制细胞的生长曲线,检测U251细胞的凋亡率。结果 对28份神经胶质瘤组织和正常组织的定量PCR分析发现,miR-592在肿瘤组织中明显低表达; miR-592过表达能明显抑制U251细胞的生长; 流式细胞分析显示,miR-592显著促进U251细胞凋亡:对照组晚期凋亡率为(7.2±0.68)%,而转染miR-592组晚期凋亡率为(17.47±1.45)%; 荧光素酶双报告实验以及蛋白免疫印迹法实验发现miR-592直接靶向Runx2的3'-UTR来抑制Runx2蛋白的表达水平。结论 miR-592通过直接靶向Runx2来诱导神经胶质瘤细胞凋亡,进而抑制细胞生长。
Abstract:
ObjectiveTo investigate the effect of miR-592 on U251 cell apoptosis in the Glioma.Methods The expression of miR-592 was analyzed by quantitative PCR in Glioma tissues from patients. Next, U251 cells were transfected with miR-592 mimics and then the growth of cells was detected by MTT assay. To extensively explore the direct target of miR-592 in glioma, dual-luciferase reporter assay and western blot assay were performed to confirm that Runx2 is the direct target of miR-592 in U251 cells. In order to test whether Runx2 was the functional target of miR-592, the cell growth curve was determined by down-regulating the level of Runx2. Moreover, the apoptosis of U251 was also detected after Runx2 knocking down.Results The expression of miR-592 was significantly reduced in glioma tissues. Over-expression miR-592 remarkably increased the apoptotic rate of U251 cells. Dual-luciferase reporter assay indicated that Runx2 was the direct target of miR-592 in U251 cells. Suppressed expression of Runx2 by siRNA prominently suppressed U251 cell growth and induced the cell apoptotic rate.Conclusion miR-592 suppressed the growth and promoted the apoptotic rate of U251 cells by targeting Runx2.

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更新日期/Last Update: 2017-08-20