[1]蒋锋,袁婕,崔小丽,等.脑血疏口服液对大鼠脑缺血再灌注损伤后血脑屏障的影响[J].卒中与神经疾病杂志,2017,24(05):423-426.[doi:10.3969/j.issn.1007-0478.2017.05.010]
　Jiang Feng,Yuan Jie,Cui Xiaoli,et al.The effects of Naoxueshu oral liquid on blood-brain barrier after cerebral ischemia-reperfusion injury in rats[J].Stroke and Nervous Diseases,2017,24(05):423-426.[doi:10.3969/j.issn.1007-0478.2017.05.010]
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脑血疏口服液对大鼠脑缺血再灌注损伤后血脑屏障的影响()
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《卒中与神经疾病》杂志[ISSN:1007-0478/CN:42-1402/R]

卷:
第24卷
期数:
2017年05期
页码:
423-426
栏目:
论 著
出版日期:
2017-10-26

文章信息/Info

Title:
The effects of Naoxueshu oral liquid on blood-brain barrier after cerebral ischemia-reperfusion injury in rats
文章编号:
1007-0478(2017)05-0423-04
作者:
蒋锋; 袁婕; 崔小丽; 王晓辉; 程仙送; 王莉; 山媛
710061 西安,陕西省人民医院神经内一科
Author(s):
Jiang Feng; Yuan Jie; Cui Xiaoli; et al.
Neurology Department Ⅰof Shanxi Province People's Hospital,xi'an 710061
关键词:
脑缺血再灌注损伤 脑血疏口服液 血脑屏障 基质金属蛋白酶-9
Keywords:
Cerebral ischemia Naoxueshu oral liquid Blood-brain barrier MMP-9
分类号:
R743.33
DOI:
10.3969/j.issn.1007-0478.2017.05.010
摘要:
目的 探讨脑血疏口服液对大鼠脑缺血再灌注损伤后血脑屏障的影响。方法 将120只SD大鼠随机分为3组:假手术组、对照组,脑血疏组; 采用线栓法建立大鼠左侧大脑中动脉闭塞再灌注模型,缺血2 h后拔出线栓,恢复灌注24 h; 采用Longa FZ 5级评分法进行大鼠神经功能缺损评分; TTC染色计算脑梗死体积百分比; 运用干-湿重法测脑含水率; 通过伊文思蓝( EB)含量反映血脑屏障的损伤程度; 免疫组化检测基质金属蛋白酶-9(MMP-9)的表达水平。结果(1)假手术组大鼠在神经功能缺损评分、脑梗死体积、脑含水率均低于对照组(P<0.01); 脑组织中EB含量和MMP-9表达水平较对照组低(P<0.01);(2)脑血疏组大鼠的神经功能缺损评分较低、脑梗死体积较小,脑水肿程度较轻; EB含量和MMP-9表达水平均较对照组明显减少(P<0.01)。结论 脑血疏口服液对大鼠脑缺血再灌注损伤后血脑屏障具有保护作用,其机制可能是通过抑制MMP-9的表达。
Abstract:
ObjectiveTo investigate the effects of Naoxuechu oral liquid on blood - brain barrier after cerebral ischemia-reperfusion injury in rats.Methods 120 SD rats were randomly divided among three groups:sham-operation group,control group and Naoxueshu group.The middle cerebral artery occlusion reperfusion model was established by thread embolism method.After 2 hours of ischemia,the suture was pulled out and the perfusion was resumed for 24h.The neurological deficit was assessed by Longa FZ 5-score while TTC staining was utilized to calculate the percentage of cerebral infarct volume.The dry-wet weight method was used to measure the water content of the brain and the level of the blood-brain barrier was reflected by Evans blue(EB)content.The expression of matrix metalloproteinase-9(MMP-9)was considered by immunohistochemistry.Results Compared with the control group,the neurological deficit score,cerebral infarct volume and cerebral edema were significantly lower in the sham-operation group(P<0.01),and the EB content and MMP-9 expression in the brain tissue were less.The difference was significant(P<0.01).Compared with the control group,the scores of neurological deficits,cerebral infarction volume and cerebral edema were lower in Naoxueshu group.The content of EB and the expression of MMP-9 was significantly lower than those in the control group.There was considerable statistical difference(P<0.01).Conclusion Naoxueshu oral Liquid has the protective effect on the blood-brain barrier after cerebral ischemia reperfusion injury in rats.The mechanism might be through inhibition of MMP-9 expression.

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更新日期/Last Update: 2017-10-20