[1]王航,宋歌,刘永建,等.自噬标志物LC3B表达水平的改变与星形细胞瘤手术患者预后的相关性研究[J].卒中与神经疾病杂志,2018,25(05):572-575+579.[doi:10.3969/j.issn.1007-0478.2018.05.019]
　Wang Hang,Song Ge,Liu Yongjian,et al.The association between altered expression level of the autophagy marker LC3B and poor prognosis in astrocytoma[J].Stroke and Nervous Diseases,2018,25(05):572-575+579.[doi:10.3969/j.issn.1007-0478.2018.05.019]
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自噬标志物LC3B表达水平的改变与星形细胞瘤手术患者预后的相关性研究()
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《卒中与神经疾病》杂志[ISSN:1007-0478/CN:42-1402/R]

卷:
第25卷
期数:
2018年05期
页码:
572-575+579
栏目:
论 著
出版日期:
2018-10-26

文章信息/Info

Title:
The association between altered expression level of the autophagy marker LC3B and poor prognosis in astrocytoma
文章编号:
1007-0478(2018)05-0572-05
作者:
王航; 宋歌; 刘永建; 谢万福
723000 陕西省汉中市人民医院神经外科(王航 宋歌 刘永建); 西安交通大学医学院第一附属医院神经外科[谢万福(通信作者)]
Author(s):
Wang Hang; Song Ge; Liu Yongjian; et al.
Department of Neurosurgery,Hanzhoung People's Hospital,Hanzhoung Shanxi 723000
关键词:
星形细胞瘤 自噬 LC3B Beclin-1
Keywords:
Astrocytoma Autophagy LC3B Beclin-1
分类号:
R739.41
DOI:
10.3969/j.issn.1007-0478.2018.05.019
文献标志码:
A
摘要:
目的 评估两种自噬标志物LC3B和Beclin-1的蛋白表达水平与星形细胞瘤患者的临床参数之间的关系。方法 回顾性收集2009年12月-2012年12月星形细胞瘤患者的106个薄切片,通过切片染色分析LC3B和Beclin-1与患者生存时间的关系。结果 发现LC3B与蛋白质表达水平与抗辐射或化学疗法显著相关(P=0.02); 高强度的LC3B染色预示着不良的预后(P=0.03); Beclin-1蛋白表达水平与患者的总体生存率不相关; CD133和LC3B高表达的患者的总生存期为38个月,CD133和LC3B两者均为弱表达的患者的总生存期为50个月,CD133和LC3B高表达的患者的生存时间明显短于CD133和LC3B低表达者(P=0.049)。结论 星形细胞瘤癌症干细胞样细胞和增强的自噬可引起对放射疗法/化学疗法的抗性,并且针对星形细胞瘤中的癌症干细胞样细胞可提供可行的治疗方法。
Abstract:
ObjectiveTo evaluate the relationship between the expression levels of two autophagy markers LC3B,Beclin-1 and the clinical parameters of astrocytoma patients.Methods A total of 106 thin sections from patients with astrocytoma from December 2009 to December 2012 were retrospectively collected.The relationship between LC3B and Beclin-1 expression levels and patient's survival time was analyzed by section staining.Results LC3B expression level was found to be correlated significantly with protein expression level and anti-radiation or chemotherapy(P=0.02).High-intensity LC3B staining predicted a poor prognosis(P=0.03).Beclin-1 protein expression level was not associated with overall patient survival.Overall survival was 38 months for CD133 and LC3B-overexpressing patients,50 months for patients with both CD133 and LC3B being poorly expressed,and significantly longer for CD133 and LC3B-overexpressing patients than that for CD133 And LC3B low expression levels(P=0.049).Conclusion Astrocytoma cancer stem cell-like cells and enhanced autophagy could cause resistance to radiotherapy / chemotherapy and provide viable therapies for cancer stem cell-like cells in astrocytoma.

参考文献/References:

[1] Louis DN,Perry A,Reifenberger G,et al.The 2016 World Health Organization classification of tumors of the central nervous system:a summary[J].Acta Neuropathol,2016,131(6):803-820.
[2] El-Sayed M,Taha MM.Immunohistochemical expression of cycloxygenase-2 in astrocytoma:correlation with angiogenesis,tumor progression and survival[J].Turk Neurosurg,2011,21(1):27-35.
[3] N BN,Donson AM,Birks DK,et al.Insulin-like growth factor 2 mRNA binding protein 3 expression is an Independent prognostic factor in pediatric pilocytic and pilomyxoid astrocytoma[J].Journal of Neuropathology&Experimental Neurology,2013,72(5):442-449.
[4] 王鹏,宋扬,高丽鹤,等.肌醇六磷酸或(和)肌醇对多种癌细胞增殖影响[J].齐鲁医学杂志,2016,31(3):301-303,306.
[5] 吴安琪,曙光,王晶,等.癌症干细胞特性及靶向治疗进展[J].转化医学电子杂志,2017,4(9):1-8.
[6] 袁凡恩,陈谦学.胶质瘤干细胞的研究进展[J].中国临床神经外科杂志,2016,21(8):508-511.
[7] 张亚,丁晓,陆海军.恶性胶质瘤靶向治疗研究进展[J].中华肿瘤防治杂志,2017,24(5):350-356.
[8] 陈志杰,石松生,陈春美.脑胶质瘤干细胞靶向免疫治疗研究进展[J].国际神经病学神经外科学杂志,2014,41(2):181-184.
[9] 冯文之,陈扬,俞立.细胞自噬分子机制的进展[J].生命科学,2015,27(7):859-866.
[10] 地里夏提·白克力,伊利夏提·肖开提,李学军.自噬在肿瘤耐药中的作用研究进展[J].国际药学研究杂志,2015,42(3):303-309+337.
[11] White E.The role for autophagy in cancer[J].Journal of Clinical Investigation,2015,125(1):42-46.
[12] Kashima J,Shintani-ishida K,Nakajima M,et al.Immunohistochemical study of the autophagy marker microtubule-associated protein 1 light chain 3 in normal and steatotic human livers[J].Hepatol Res,2014,44(7):779-787.
[13] Tanida I,Ueno T,Uchiyama Y.A super-ecliptic,pHluorin-mKate2,tandem fluorescent protein-tagged human LC3 for the monitoring of mammalian autophagy[J].PLoS One,2014,9(10):e110600.
[14] Wei Y,Zou Z,Becker N,et al.EGFR-mediated Beclin 1 phosphorylation in autophagy suppression,tumor progression,and tumor chemoresistance[J].Cell,2013,154(6):1269-1284.
[15] Fu LL,Cheng Y,Liu B.Beclin-1:autophagic regulator and therapeutic target in cancer[J].International Journal of Biochemistry & Cell Biology,2013,45(5):921-924.
[16] Chen S,Jiang YZ,Huang L,et al.The residual tumor autophagy marker LC3B serves as a prognostic marker in local advanced breast cancer after neoadjuvant chemotherapy[J].Clin Cancer Res,2013,19(24):6853-6862.
[17] Auffinger B,Tobias AL,Han Y,et al.Conversion of differentiated cancer cells into cancer stem-like cells in a glioblastoma model after primary chemotherapy[J].Cell Death Differ,2014,21(7):1119-1131.
[18] Bao B,Ali S,Ahmad A,et al.Differentially expressed miRNAs in cancer-stem-like cells:markers for tumor cell aggressiveness of pancreatic cancer[J].Stem Cells Dev,2014,23(16):1947-1958.
[19] Huang X,Bai HM,Chen L,et al.Reduced expression of LC3B-II and Beclin 1 in glioblastoma multiforme indicates a down-regulated autophagic capacity that relates to the progression of astrocytic tumors[J].J Clin Neurosci,2010,17(12):1515-1519.
[20] Melguizo C,Prados J,González B,et al.MGMT promoter methylation status and MGMT and CD133 immunohistochemical expression as prognostic markers in glioblastoma patients treated with temozolomide plus radiotherapy[J].J Transl Med,2012,10(1):250.
[21] 张琼霞,王希成,陈树鹏,等.干细胞标志物ALDH1与大样本乳腺癌患者的预后关系[J].临床医学工程,2016,23(12):1585-1587.
[22] 李丽娟,温树信,王斌全,等.肿瘤干细胞标记物USP22蛋白在喉鳞状细胞癌中的表达及预后分析[J].中华耳鼻咽喉头颈外科杂志,2014,49(6):479-482.
[23] 杨丽萍,侯俊德,段爱红,等.肿瘤干细胞标志物CD133和Nestin在卵巢癌中的表达及意义[J].临床和实验医学杂志,2015,14(4):301-304.
[24] 李式浩,徐培坤,李显雄,等.术后辅助不同放化疗方案对高级别胶质瘤患者的预后分析[J].安徽医药,2017,21(10):1858-1860.
[25] Mrugala MM.Advances and challenges in the treatment of glioblastoma:a clinician's perspective[J].Discov Med,2013,15(83):221-230.
[26] 张春,李清漪,董海影,等.PI3K/AKT/mTOR信号通路在诱导胶质瘤细胞凋亡中的作用[J].中国新药杂志,2016,25(16):1909-1912.
[27] 黄丽娟,王剑松,王海峰.自噬机制在肿瘤化疗中的研究进展[J].医学与哲学:B,2017,38(02):70-72+90.

更新日期/Last Update: 2018-10-20