[1]张婷 徐裕 薛鑫诚 何佳 高恒 王其平.长链非编码RNA MEG3、HOTAIR和MALAT-1在垂体腺瘤中的表达水平及其与肿瘤侵袭的关系[J].卒中与神经疾病杂志,2019,26(02):209-213.[doi:10.3969/j.issn.1007-0478.2019.02.019]
　Zhang Ting*,Xu Yu,Xue Xincheng,et al.The expression levels of long non-coding RNA MEG3,HOTAIR and MALAT-1 in pituitary adenomas and its relationship with tumor invasion[J].Stroke and Nervous Diseases,2019,26(02):209-213.[doi:10.3969/j.issn.1007-0478.2019.02.019]
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长链非编码RNA MEG3、HOTAIR和MALAT-1在垂体腺瘤中的表达水平及其与肿瘤侵袭的关系()
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《卒中与神经疾病》杂志[ISSN:1007-0478/CN:42-1402/R]

卷:
第26卷
期数:
2019年02期
页码:
209-213
栏目:
论 著
出版日期:
2019-04-20

文章信息/Info

Title:
The expression levels of long non-coding RNA MEG3,HOTAIR and MALAT-1 in pituitary adenomas and its relationship with tumor invasion
文章编号:
1007-0478(2019)02-0209-05
作者:
张婷 徐裕 薛鑫诚 何佳 高恒 王其平
210400 江苏省江阴市东南大学医学院附属江阴医院中心实验室(张婷),神经外科[徐裕 薛鑫诚 何佳 高恒 王其平(通信作者)]
Author(s):
Zhang Ting*; Xu Yu; Xue Xincheng; et al.*
Central Laboratory,the Affiliated Jiangyin Hospital of Southeast University Medical College,Jiangyin Jiangsu 214400
关键词:
垂体腺瘤 长链非编码RNA MEG3 HOTAIR MALAT-1 侵袭
Keywords:
Pituitary adenomas lncRNA MEG3 HOTAIR MALAT-1 Invasion
分类号:
R736.4
DOI:
10.3969/j.issn.1007-0478.2019.02.019
文献标志码:
A
摘要:
目的 探讨长链非编码RNA MEG3,HOTAIR和MALAT-1对垂体腺瘤发生发展和侵袭的作用。方法 应用实时定量逆转录聚合酶链式反应(qRT-PCR)检测MEG3,HOTAIR,MALAT-1在正常脑组织、非侵袭性和侵袭性垂体腺瘤中的表达情况,并分析3组之间的表达水平差异。结果 MEG3表达水平在正常脑组织、非侵袭性垂体腺瘤和侵袭性垂体腺瘤中逐渐降低,而HOTAIR表达水平逐渐升高(P<0.05)。MALAT-1的表达水平在3组样本之间没有统计学差异(P>0.05)。MEG3的表达水平与垂体腺瘤侵袭性生物学行为有相关(P<0.05),HOTAIR的表达水平与侵袭性生物学行为和肿瘤大小有关(P<0.05),而MALAT-1的表达水平与年龄、性别、分类、生物学行为和肿瘤大小之间没有显著关系(P>0.05)。结论 MEG3和HOTAIR的表达水平异常可能与垂体腺瘤的发展和侵袭密切有关。
Abstract:
ObjectiveTo study the roles of long non-coding RNA MEG3,HOTAIR and MALAT-1 in the development and invasion of pituitary adenomas.Methods Real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR)was used to detect the expression levels of MEG3,HOTAIR and MALAT-1 in normal brain tissues,non-invasive and invasive pituitary adenomas,and the expression level differences were analyzed among them.Results The expression level of MEG3 was gradually decreased from normal brain tissues to non-invasive pituitary adenomas,from non-invasive pituitary adenomas to invasive pituitary adenomas,while HOTAIR lncRNA levels were gradually increased with statistically significant difference(P<0.05).The expression level of MALAT-1 was not statistically different among the three groups(P>0.05).There was a significant association between expression level of MEG3and biological behavior of tumor invasion(P<0.05).The expression level of HOTAIR was correlated with invasive biological behavior and tumor size(P<0.05),but there was no significant correlation between the expression level of MALAT-1 and age,sex,classification,biological behavior and tumor size(P>0.05).Conclusion The abnormal expression levels of MEG3 and HOTAIR might be closely related to the development and invasion of pituitary adenomas.

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备注/Memo

备注/Memo:
基金项目:无锡市卫生计生委项目(MS201641); 江阴市卫生局科研立项(Z201401) (2018-07-12收稿)
更新日期/Last Update: 2019-04-20