[1]董慧敏 吕龙琴 董若辰 刘宝辉 毛善平.RhoE在小鼠中枢神经系统通过NF-κB信号通路调节神经细胞凋亡[J].卒中与神经疾病杂志,2019,26(05):509-512.[doi:10.3969/j.issn.1007-0478.2019.05.001]
 Dong Huimin,Lv Longqin,Dong Ruochen,et al.The RhoE regulated neurocytes apoptosis through NF-κB signaling pathway in central nervous system[J].Stroke and Nervous Diseases,2019,26(05):509-512.[doi:10.3969/j.issn.1007-0478.2019.05.001]
点击复制

RhoE在小鼠中枢神经系统通过NF-κB信号通路调节神经细胞凋亡()
分享到:

《卒中与神经疾病》杂志[ISSN:1007-0478/CN:42-1402/R]

卷:
第26卷
期数:
2019年05期
页码:
509-512
栏目:
论 著
出版日期:
2019-10-20

文章信息/Info

Title:
The RhoE regulated neurocytes apoptosis through NF-κB signaling pathway in central nervous system
文章编号:
1007-0478(2019)05-0509-05
作者:
董慧敏 吕龙琴 董若辰 刘宝辉 毛善平
430060 武汉大学人民医院神经内科[董慧敏 吕龙琴 董若辰 毛善平(通信作者)],神经外科(刘宝辉)
Author(s):
Dong Huimin Lv Longqin Dong Ruochen et al.
Department of Neurology, Renmin Hospital of Wuhan University, Wuhan 430060
关键词:
RhoE NF-κB信号通路 神经元 凋亡
Keywords:
RhoE NF-κB signaling pathway Neurocytes Apoptosis
分类号:
R741.02
DOI:
10.3969/j.issn.1007-0478.2019.05.001
文献标志码:
A
摘要:
目的 探讨RhoE对神经细胞凋亡的作用及可能的相关机制。方法 用免疫组织化学染色、Q-PCR和免疫印迹等方法检测RhoE-/-及RhoE+/+小鼠脑组织内RhoE、Caspase3和P65蛋白表达水平。结果 RhoE-/-小鼠脑组织内无RhoE蛋白表达; RhoE-/-小鼠多部位脑组织cleaved-Caspase3表达水平较RhoE+/+小鼠显著降低(P<0.05); 同时RhoE-/-小鼠脑组织内P65蛋白表达水平较RhoE+/+小鼠显著增高(P<0.05),但mRNA水平无明显改变(P>0.05)。结论 RhoE在小鼠中枢神经系统内可能通过NF-κB信号通路中P65蛋白表达水平来调控神经元的凋亡; RhoE可能是一个新的神经元凋亡调控位点。
Abstract:
ObjectiveTo explore the effect and mechanism of the RhoE on neurocytes apoptosis.Methods Immunohistochemistry,Q-PCR and western blot were performed to explore RhoE,P65and cleaved-Caspase3's expression levels in RhoE-/- and RhoE+/+mice brain tissue.Results Immunohistochemistry showed there was no expression of RhoE in RhoE-/- mice brain.Compared with RhoE+/+ mice,cleaved-Caspase3 protein level decreased in RhoE-/- mice's brain tissue(P<0.05).On the other hand,P65 protein level increased in RhoE-/- mice's brain(P<0.05),but there's no significant difference in the mRNA level(P>0.05).Conclusion The results showed the RhoE could regulated neurocytes apoptosis through P65 expression level in central nervous system.The RhoE probably regulated P65 in protein level.And the RhoE could be a new regulatory site for neurocytes apoptosis.

参考文献/References:

[1] Yang X,Wang T,Lin X,et al.Genetic deletion of RhoE/RhoE results in mouse heart Calcium leakage through upregulation of protein kinase A signaling[J].Circ Res,2015,116(1):e1-e10. [2] Gao C,Chang P,Yang L,et al.Neuroprotective effects of Hydrogen sulfide on Sodium azide-induced oxidative stress in PC12 cells[J].Int J Mol Med,2018,41(1):242-250. [3] Aliev G,Ashraf GM,Tarasov VV,et al.Alzheimer disease - future therapy based on dendrimers[J].Curr Neuropharmacol,2019,17(3):288-294. [4] Ao L Y,Yan YY,Zhou L,et al.Immune Cells After Ischemic Stroke Onset: Roles,Migration,and Target Intervention[J].J Mol Neurosci,2018,66(3):342-355. [5] Liu Y,Zhang Y,Zheng X,et al.And synaptic plasticity impairments induced by lipopolysaccharide in mice[J].J Neuroinflammation,2018,15(1):112. [6] Liu F,Liu TW,Kang J.The role of NF-kappa B-mediated JNK pathway in cognitive impairment in a rat model of sleep apnea[J].J Thorac Dis,2018,10(12):6921-6931. [7] Kaltschmidt C,Kaltschmidt B,Baeuerle PA.Brain synapses contain inducible forms of the transcription factor NF-kappa B[J].Mechanisms of Development,1993,43(2-3):135-147. [8] Meberg PJ,Kinney WR,Valcourt EG,et al.Gene expression of the transcription factor NF-kappa B in hippocampus: regulation by synaptic activity[J].Brain research.Molecular Brain Research,1996,38(2):179-190. [9] Bhakar A L,Tannis L L,Zeindler C,et al.Constitutive nuclear factor-kappa B activity is required for central neuron survival[J].J Neurosci,2002,22(19):8466-8475. [10] Chiarugi,A.Characterization of the molecular events following impairment of NF-kappaB-driven transcription in neurons[J].Brain Res Mol Brain Res,2002,109(1/2):179-188. [11] Maggirwar SB,Sarmiere PD,Dewhurst S,et al.Nerve growth factor-dependent activation of NF-kappaB contributes to survival of sympathetic neurons[J].J Neurosci,1998,18(24):10356-10365. [12] Middleton G,Hamanoue M,Enokido Y,et al.Cytokine-induced nuclear factor kappa B activation promotes the survival of developing neurons[J].J Cell Biol,2000,14(2):325-332. [13] Siebenlist U,Franzoso G,Brown K.Structure,regulation and function of NF-kappa B[J].Annual Review of Cell Biology,1994,10:405-455. [14] Dejardin E.The alternative NF-kappa B pathway from biochemistry to biology: Pitfalls and promises for future drug development[J].Biochem Pharmacol,2006,72(9):1161-1179. [15] Hayden MS,Ghosh S.Shared principles in NF-kappaB signaling[J].Cell,2008,132(3):344-362. [16] Bonizzi G,Karin M.The two NF-kappaB activation pathways and their role in innate and adaptive immunity[J].Trends Immunol,2004,25(6):280-288. [17] Wherlock M,Mellor H.The Rho GTPase family: a Racs to Wrchs story[J].J Cell Sci,2002,115(2):239-240.[18] Foster R,Hu K Q,Lu Y,et al.Identification of a novel human Rho protein with unusual properties: GTPase deficiency and in vivo farnesylation[J].Molecular and Cellular Biology,1996,16(6):2689-2699. [19] Jie W,Andrade KC,Lin X,et al.Pathophysiological functions of Rnd3/RhoE[J].Compr Physiol,2016,6(1):169-186. [20] Yue X,Yang X,Lin X,et al.RhoE haploinsufficient mice are predisposed to hemodynamic stress and develop apoptotic cardiomyopathy with heart failure[J].Cell Death & Disease,2014,5: e1284. [21] Pacary E,Heng JL,Azzarelli R,et al.Proneural transcription factors regulate different steps of cortical neuron migration through Rnd-Mediated inhibition of RhoA signaling[J].Neuron,2011,69(6):1069-1084. [22] Lin X,Liu B,Yang X,et al.Genetic deletion of RhoE results in aqueductal stenosis leading to hydrocephalus through up-regulation of Notch signaling[J].Proceedings of the National Academy of Sciences of the United States of America 2013,110(20):8236-8241. [23] Dong H,Lin X,Li Y,et al.Genetic deletion of RhoE in neural stem cells promotes proliferation via upregulation of Notch signaling[J].Oncotarget,2017,8(53):91112-91122.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(编号为81502175,81371390)(2019-04-16收稿)
更新日期/Last Update: 2019-10-20