[1]孟开顺 宋科秀 王小蕊.miRNA-368在糖尿病脑病中的表达水平变化及临床意义[J].卒中与神经疾病杂志,2019,26(06):706-709.[doi:10.3969/j.issn.1007-0478.2019.06.015]
　Meng Kaishun,Song Kexiu,Wang Xiaorui..Expression level change of miRNA-368 in patients with diabetic encephalopathy and its clinic significance[J].Stroke and Nervous Diseases,2019,26(06):706-709.[doi:10.3969/j.issn.1007-0478.2019.06.015]
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miRNA-368在糖尿病脑病中的表达水平变化及临床意义()
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《卒中与神经疾病》杂志[ISSN:1007-0478/CN:42-1402/R]

卷:
第26卷
期数:
2019年06期
页码:
706-709
栏目:
论 著
出版日期:
2019-12-25

文章信息/Info

Title:
Expression level change of miRNA-368 in patients with diabetic encephalopathy and its clinic significance
文章编号:
1007-0478(2019)06-0706-04
作者:
孟开顺 宋科秀 王小蕊
572000 海南省三亚市人民医院
Author(s):
Meng Kaishun;  Song Kexiu;  Wang Xiaorui.
Sanya City People 's Hospital，Sanya 572000
关键词:
三亚地区 外周血糖尿病 脑病循环 miRNAmiRNA-368增殖细胞凋亡
Keywords:
Sanya areaPeripheral bloodDiabetic encephalopathyCirculating miRNAmiRNA-368ProliferationApoptosis
分类号:
R742
DOI:
10.3969/j.issn.1007-0478.2019.06.015
文献标志码:
A
摘要:
目的 探讨糖尿病脑病患者外周血血清和单核细胞中miRNA-368水平的表达变化及临床意义。方法qRT-PCR检测糖尿病脑病中患者外周血血清的miRNA-368表达水平，并将miRNA-368转染到糖尿病脑患者外周血单核细胞中，然后通过qRT-PCR检测转染效率，采取CCK-8和克隆形成实验检测miRNA-368对糖尿病脑患者外周血单核细胞增殖的影响，流式细胞技术检测miRNA-368对糖尿病脑患者外周血单核细胞周期和凋亡率的影响。结果27例糖尿病脑病患者外周血中miRNA-368的相对表达量为1.402，95%CI=0.363～0.595；miRNA-368相对表达水平与患者的一般资料如年龄、性别并不存在明显关系（P＞0.05）；糖尿病脑病患者的miRNA-368相对表达水平明显上调(P<0.05)；qRT-PCR显示糖尿病脑病外周血单核细胞miRNA-368的表达水平明显上调；si-miRNA-368干预后糖尿病脑病患者外周血单核细胞增殖受到抑制，凋亡率增高。 结论miRNA-368在糖尿病脑病患者外周血血清和单核细胞中的表达水平明显上调，且miRNA-368能抑制单核细胞的凋亡，促进了糖尿病脑病的发生发展。
Abstract:
Objective To study the expression level change and clinic significance of miRNA-368 in peripheral blood of patients with diabetic encephalopathy. Methods qRT-PCR was used to detect the expression level of miRNA-368 in peripheral blood of patients with diabetic encephalopathy in Sanya. MiRNA-368 was transfected into peripheral blood mononuclear cells collected from patients. The transfection efficiency was detected by qRT-PCR. The effect of miRNA-368 on the proliferation of peripheral blood mononuclear cells in diabetic encephalopathy patients was detected by CCK-8 and clonogenicity assay. The effect of miRNA-368 on the peripheral blood mononuclear cell cycle and apoptotic rate in diabetic encephalopathy patients was analyzed by flow cytometry. Results The relative expression level of miRNA-368 in 27 patients with diabetic encephalopathy was 1.402, and the 95% CI was 0.363~0.595. The relative expression level of miRNA-368 was not related to the general data of patients such as age and gender (P> 0.05), while the relative expression level of miRNA-368 in diabetic encephalopathy patients was significantly increased (P<0.05). The relative expression level of miRNA-368 was significantly correlated with that of diabetic encephalopathy. QRT-PCR showed that miRNA-368 expression level was significantly up-regulated in the serum and peripheral blood mononuclear cells of patients with diabetic encephalopathy. After the intervention of si-miRNA-368, the proliferation of peripheral blood mononuclear cells in diabetic encephalopathypatients was inhibited and the apoptosis rate increased. Conclusion The expression level of miRNA-368 in the serum and peripheral blood mononuclear cells of diabetic encephalopathy patients was significantly upregulated, and miRNA-368 could inhibit the apoptosis of blood mononuclear cells in patients with diabetic encephalopathy, which might promote the development of diabetic encephalopathy.

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备注/Memo

备注/Memo:
基金项目：国家“十一五”科技支撑重大专项基金资助项目(2006BAI04A02)；四川省应用基础研究计划项目（2014JC0166）；四川省卫生厅资助项目（110368）
更新日期/Last Update: 2019-12-25