[1]高冠群 方婧 王岩 张卓伯 邵海艳.血管内皮生长因子基因修饰诱导多能干细胞的生物学特性的研究[J].卒中与神经疾病杂志,2020,27(01):24-27.[doi:10.3969/j.issn.1007-0478.2020.01.005]
　Gao Guanqun,Fang Jing,Wang Yan,et al.Biological characteristics of the induced pluripotent stem cells modified by vascular endothelial growth factor gene。 Department of Neurology, The Fourth affiliated Hospital of Harbin Medical University, Harbin 150001[J].Stroke and Nervous Diseases,2020,27(01):24-27.[doi:10.3969/j.issn.1007-0478.2020.01.005]
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血管内皮生长因子基因修饰诱导多能干细胞的生物学特性的研究()
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《卒中与神经疾病》杂志[ISSN:1007-0478/CN:42-1402/R]

卷:
第27卷
期数:
2020年01期
页码:
24-27
栏目:
论 著
出版日期:
2020-01-30

文章信息/Info

Title:
Biological characteristics of the induced pluripotent stem cells modified by vascular endothelial growth factor gene。 Department of Neurology, The Fourth affiliated Hospital of Harbin Medical University, Harbin 150001
文章编号:
1007-0478(2020)01-0024-05
作者:
高冠群 方婧 王岩 张卓伯 邵海艳
150001 哈尔滨医科大学附属第四医院神经内科; 哈尔滨市第一医院神经内科[邵海艳(硕士研究生,通信作者)]
Author(s):
Gao Guanqun;  Fang Jing;  Wang Yan; et al.
Department of Neurology, The Fourth affiliated Hospital of Harbin Medical University, Harbin 150001
关键词:
诱导多能干细胞 血管内皮生长因子 转染 基因表达
Keywords:
Induced pluripotent stem cells Vascular endothelial growth factor Transfection Gene expression
分类号:
R741
DOI:
10.3969/j.issn.1007-0478.2020.01.005
文献标志码:
A
摘要:
目的 探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)基因修饰诱导多能干细胞(induced pluripotent stem cells,iPS)的生物学特性。方法 将培养好的iPS细胞分为3组,即N0(纯iPS培养)组、N1(空腺病毒载体转染iPS)组、N2(转染VEGF基因的iPS)组; 3组细胞分别进行传代培养,并采用MTT方法检测转染VEGF基因对细胞增殖的影响; 采用酶联免疫(ELISA)方法检测细胞培养上清液中VEGF的表达水平。结果 转染VEGF基因对iPS细胞增殖没有明显影响; N2组iPS细胞分泌的VEGF蛋白表达水平较N1组显著增加(P<0.05)。结论 经VEGF基因转染的iPS可正常存活生长,并可稳定表达该基因。
Abstract:
ObjectiveTo explore biological characteristics of the induced pluripotent stem cells(iPS)modified by vascular endothelial growth factor(VEGF)gene.Methods The cultured iPS cells were divided into three groups:N0(pure iPS culture)group,N1(empty adenovirus vector transfection iPS)group,N2(iPS group transfected with VEGF gene)group.Three groups of cells were subcultured respectively, and MTT method was determined to detect transfection VEGF gene effects on cell proliferation. The enzyme-linked immune(ELISA)method was used to detect the expression level of VEGF in cell culture supernatant on content.Results The cell proliferation of the transferred iPS was similar to those of the N0(pure iPS culture)group, N1(empty adenovirus vector transfection iPS)group. The level of VEGF protein in supernatant after VEGF gene transfection was significantly higher than that of the N1(empty adenovirus vector transfection iPS)group(P<0.05).Conclusion VEGF gene transfection iPS could grow normally and stably express the gene.

参考文献/References:

[1] Takahashi K,Yamanaka S.Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors[J].Cell,2006,126(4):663-676.
[2]Yu JY,Vodyanik MA,Smuga-Otto KA,et al.Induced pluripotent stem cell lines derived from human somatic cells[J].Science(80- ),2007,318(5858):1917-1920.
[3]Lisa S Z,Cameron J K,AmitMaity Deirdre P M,et al.Hypoxia and VEGF mRNA expression in human tumors[J].Neoplasia,2001,3(6):500-508.
[4]Pereira Lopes FR,Lisboa B,Frattini F,et al.Enhancement of sciatic nerve regeneration after vascular endothelial growth factor(VEGF)gene therapy[J].Neuropathol Appl Neurobiol,2011,37(6):600-612.
[5]Kim OJ,Hong SH,Oh SH,et al.Association between VEGF polymorphisms and homocysteine levels in patients with ischemic stroke and silent brain infarction[J].Stroke,2011,42(9):2U73-2393.
[6]Guan WH,Somanath PR,Kozak A,et al.Vascular protection by angiotensin receptor antagonism involves differential VEGF expression in both hemispheres after experimental stroke[J].PLoS One,2011,6(9):524-551.
[7]Michalis A,SerafimKaltsas,Konstantinos N.Syrigos.the VEGF pathway in lung cancer[J].Cancer Chemother Pharmacol,2013,72(6):415-442.
[8]Cuijpers Y,Harro VL.Early diagnostics and Alzheimer's disease:Beyond cure and care[J].Technological Forecasting & Social Change,2014,24(4):415-423.
[9]Huangfu D,Osafune K,Maehr R,et al.Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2[J].Nat Biotechnol,2008,26(11):1269-1275.
[10]Zhou T,Benda C,Dunzinger S,et al.Generation of human induced pluripotent stem cells from urine samples[J].Nat Protoc,2012,7(12):2080-2089.
[11]Wu SM,Hochedlinger K.Harnessing the potential of induced pluripotent stem cells for regenerative medicine[J].Nat Cell Biol,2011,13(5):497-505.
[12]Liu GH,Qu J,Suzuki K,et al.Progressive degeneration of human neural stem cells caused by pathogenic LRRK2[J].Nature,2012,491(7425):603-607.
[13]Schwartz RE,Trehan K,Andrus L,et al.Modeling hepatitis C virus infection using human induced pluripotent stem cells[J].Proc Natl Acad Sci U S A,2012,109(7):2544-2548.
[14]Takebe T,Sekine K,Enomura M,et al.Vascularized and functional human liver from an iPSC-derived organ bud transplant[J].Nature,2013,499(7459):481-484.
[15]Yan BB,Abdelli LS,Singla DK.Transplanted induced pluripotent stem cells improve cardiac function and induce neovascularization in the infarcted hearts of db/db mice[J].Mol Pharm,2011,8(5):1602-1610.
[16]Lu TY,Lin B,Kim J,et al.Repopulation of decellularized mouse heart with human induced pluripotent stem cell-derived cardiovascular progenitor cells[J].Nat Commun,2013,4(1):2307.
[17]Lu X,Mazur SJ,Lin T,et al.The pluripotency factor nanog promotes breast cancer tumorigenesis and metastasis[J].Oncogene,2014,33(20):2655-2664.
[18]Guha P,Morgan JW,Mostoslavsky GA,et al.Lack of immune response to differentiated cells derived from syngeneic induced pluripotent stem cells(vol 12, pg 407, 2013)[J].Cell Stem Cell,2017,21(1):144-148.
[19]Banito A,Gil J.Induced pluripotent stem cells and senescence: learning the biology to improve the technology[J].EMBO Rep,2010,11(5):353-359.
[20]Bass AJ,Watanabe H,Mermel CH,et al.SOX2 is an amplified lineage-survival oncogene in lung and esophageal squamous cell carcinomas[J].Nat Genet,2009,41(11):1238-1242.

备注/Memo

备注/Memo:
基金项目:黑龙江省教育厅资助项目(编号为12541539)(2019-05-10收稿)
更新日期/Last Update: 2020-01-30