[1]程安林 寿记新.血浆miR-424,FGF2水平与急性缺血性脑卒中患者血管介入术后出血转化的相关性分析[J].卒中与神经疾病杂志,2021,28(02):153-157.[doi:10.3969/j.issn.1007-0478.2021.02.004]
　Chen Anlin*,Shou Jixin..The correlation between plasma miR-424 and FGF2 levels and hemorrhagic transformation in patients with acute ischemic stroke after vascular intervention[J].Stroke and Nervous Diseases,2021,28(02):153-157.[doi:10.3969/j.issn.1007-0478.2021.02.004]
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血浆miR-424,FGF2水平与急性缺血性脑卒中患者血管介入术后出血转化的相关性分析()
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《卒中与神经疾病》杂志[ISSN:1007-0478/CN:42-1402/R]

卷:
第28卷
期数:
2021年02期
页码:
153-157
栏目:
论 著
出版日期:
2021-04-20

文章信息/Info

Title:
The correlation between plasma miR-424 and FGF2 levels and hemorrhagic transformation in patients with acute ischemic stroke after vascular intervention
文章编号:
1007-0478(2021)02-0153-05
作者:
程安林 寿记新
459000 河南省济源市中医院神经外科(程安林); 郑州大学第五附属医院神经外科(寿记新)
Author(s):
Chen Anlin*;  Shou Jixin.
*Department of Neurosurgery, Jiyuan Hospital of Traditional Chinese Medicine. Jiyuan Henan 459000
关键词:
微小RNA-424 成纤维细胞生长因子2 急性缺血性脑卒中 出血转化 相关性
Keywords:
MicroRNA-424 Fibroblast growth factor 2 Acute ischemic stroke Hemorrhagic transformation Correlation
分类号:
R743.3
DOI:
10.3969/j.issn.1007-0478.2021.02.004
文献标志码:
A
摘要:
目的 探讨血浆微小RNA-424(MicroRNA,miR-424)、成纤维细胞生长因子2(Fibroblast growth factor 2,FGF2)水平与急性缺血性脑卒中(Acute ischemic stroke,AIS)患者血管介入术后出血转化(Hemorrhagic transformation,HT)的相关性。方法 选取2018年12月-2020年3月本院收治的AIS患者104例为研究对象,均行脑部血管机械取栓,根据术后患者头颅计算机X线断层扫描(Computed tomography,CT)复查表现将其分为HT组28例,非HT组76例,收集患者一般资料及入院时美国国立卫生研究院卒中量表(National institutes of health stroke scale,NIHSS)评分,采集患者术前及术后血浆样本,利用荧光定量聚合酶链反应(Polymerase chain reaction,PCR)技术检测血浆miR-424,FGF2 mRNA水平; Pearson法分析AIS发生HT患者血浆miR-424,FGF2水平的相关性; 利用多因素Logistic回归分析AIS患者血管介入术后HT的危险因素; 利用受试者工作特征曲线(Receiver operator characteristic curve,ROC)评价血浆miR-424,FGF2水平对AIS患者血管介入术后HT的预测价值。结果 HT组入院时NIHSS评分高于非HT组(P<0.05)。与非HT组相比较,术前HT组患者miR-424水平呈低表达(P<0.05),FGF2 mRNA水平呈高表达(P<0.05); 与术前比较,术后HT组和非HT组血浆miR-424水平均升高,且HT组低于非HT组; FGF2 mRNA水平均降低,且HT组高于非HT组(P均<0.05)。HT组血浆miR-424与FGF2水平呈负相关(r=-0.629,P<0.05)。多因素Logistic回归分析显示,入院NIHSS评分高、miR-424水平低、FGF2水平高是AIS患者血管介入术后HT的独立危险因素(P<0.05)。ROC显示,术前血浆miR-424,FGF2水平预测AIS患者发生HT的曲线下面积(Area of the under curve,AUC)分别为0.796、0.820,二者联合预测的AUC为0.905,敏感性和特异性分别为71.43%、94.74%。结论 血管介入术后发生HT的AIS患者血浆miR-424水平低、FGF2水平高,对AIS患者术后HT有一定的预测价值,有助于预后评估。
Abstract:
ObjectiveTo investigate the correlation between plasma microRNA-424(miR-424), fibroblast growth factor 2(FGF2)levels, and hemorrhagic transformation(HT)after vascular intervention in patients with acute ischemic stroke(AIS).Methods From December 2018 to March 2020, we enrolled 104 patients with AIS who underwent mechanical thrombectomy. The patients were divided into HT group(n=28)and non HT group(n=76)according to CT examination after operation. The general information and National Institutes of Health Stroke Scale(NIHSS)score at admission were collected. Plasma samples were collected before and after operation. The levels of miR-424 and FGF2 mRNA in plasma were detected by fluorescence quantitative PCR. Pearson method was used to analyze the correlation between plasma miR-424 and FGF2 levels in patients with HT. Multivariate Logistic regression was used to analyze the risk factors of HT in AIS patients. The receiver operating characteristic(ROC)curve was used to evaluate the predictive value of plasma miR-424 and FGF2 levels for HT in patients with AIS after vascular intervention.Results In the HT group, the NIHSS score at admission was higher than that of the non-HT group(P<0.05). Before operation, the expression of miR-424 was lower, while the expression of FGF2 was higher in the HT group than those in the non-HT group(P<0.05). Compared with those before operation, the plasma miR-424 in HT group and non-HT group after operation were increased, and that in the HT group was lower than that in the non-HT group. The FGF2 mRNA after operation was increased than before operation, and that in the HT group was higher than that in the non-HT group(P<0.05). Plasma miR-424 and FGF2 were negatively correlated with each other in the HT group(r=-0.629, P<0.05). Multivariate Logistic analysis showed that high NIHSS score at admission, low miR-424 level, and high FGF2 level were independent risk factors for HT in AIS patients after the vascular intervention(P<0.05). ROC results showed that the area under the curve(AUC)of preoperative plasma miR-424 and FGF2 in predicting HT in AIS patients was 0.796 and 0.820, respectively. The AUC predicted by the two methods was 0.905, and the sensitivity and specificity were 71.43% and 94.74%, respectively.Conclusion The plasma level of miR-424 in AIS patients with HT after vascular intervention is low, while the level of FGF2 is high. The predictive value for postoperative HT in AIS patients, and are helpful to evaluate the prognosis.

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备注/Memo

备注/Memo:
基金项目:2018年度河南省医学科技攻关计划项目(2018020445)
更新日期/Last Update: 2021-04-20