[1]刘聪聪,孟兰霞,张振涛.突触蛋白I片段促进α-突触核蛋白的聚集[J].卒中与神经疾病杂志,2023,30(01):3-8.[doi:10.3969/j.issn.1007-0478.2023.01.001]
　Liu Congcong,Meng Lanxia,Zhang Zhentao..Synapsin I fragment promotes α-synuclein aggregation[J].Stroke and Nervous Diseases,2023,30(01):3-8.[doi:10.3969/j.issn.1007-0478.2023.01.001]
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突触蛋白I片段促进α-突触核蛋白的聚集()
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《卒中与神经疾病》杂志[ISSN:1007-0478/CN:42-1402/R]

卷:
第30卷
期数:
2023年01期
页码:
3-8
栏目:
论著
出版日期:
2023-03-20

文章信息/Info

Title:
Synapsin I fragment promotes α-synuclein aggregation
文章编号:
1007-0478(2023)01-0003-06
作者:
刘聪聪; 孟兰霞; 张振涛
430060 武汉大学人民医院神经内科[刘聪聪 孟兰霞 张振涛(通信作者)]
Author(s):
Liu Congcong;  Meng Lanxia;  Zhang Zhentao.
Department of Neurology, Renmin Hospital of Wuhan University, Wuhan 430060
关键词:
突触蛋白I 天冬酰胺内肽酶 α-突触核蛋白 阿尔茨海默病
Keywords:
Synapsin I Asparagine endopeptidase α-synuclein Alzheimer's disease
分类号:
R742
DOI:
10.3969/j.issn.1007-0478.2023.01.001
文献标志码:
A
摘要:
目的 探讨突触蛋白I(Synapsin I,Syn I)被剪切后形成的C83片段对α-突触核蛋白聚集的影响。方法 在稳定表达α-突触核蛋白的HEK293细胞、小鼠原代神经元以及Tau P301S转基因小鼠体内分别过表达Syn I全长及其C83片段,通过细胞免疫荧光染色、蛋白免疫印迹技术以及免疫组织化学染色的方法观察Syn I全长及其C83片段对α-突触核蛋白磷酸化及聚集的影响。结果 Syn I C83片段促进HEK293细胞中α-突触核蛋白的磷酸化、泛素化以及聚集,并诱导小鼠原代神经元以及Tau P301S转基因小鼠体内α-突触核蛋白的磷酸化和聚集。结论 在细胞和动物模型中Syn I C83片段均可以促进α-突触核蛋白的聚集,这可能是Syn I C83片段导致认知功能障碍的重要原因之一。
Abstract:
ObjectiveTo investigate the effect of synapsin I fragment(Syn I C83)on α-synuclein aggregation.Methods The synapsin I and Syn I C83 fragment were overexpressed in HEK293 cells stably expression α-synuclein, primary mouse neurons, and Tau P301S transgenic mice. The effects of synapsin I and Syn I C83 fragment on α-synuclein phosphorylation and aggregation were observed by immunofluorescence staining, Western blot, and immunohistochemical staining.Results Syn I C83 fragment promotes α-synuclein phosphorylation, ubiquitination, and aggregation in HEK293 cells. Syn I C83 fragment also induces α-synuclein phosphorylation and aggregation in primary neurons and Tau P301S transgenic mice.Conclusion Syn I C83 fragment promoted the aggregation of α-synuclein in cellular and animal models. This may contribute to cognitive impairment mediated by Syn I C83 fragment.

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备注/Memo

备注/Memo:
基金项目:国家重点研发计划(No. 2019YFE0115900)
更新日期/Last Update: 2023-03-20