[1]窦浩瑜〓朱彧〓周为.环境内分泌干扰物急性暴露对大鼠海马组织蛋白表达水平的影响[J].卒中与神经疾病杂志,2023,30(03):294-296,306.[doi:10.3969/j.issn.1007-0478.2023.03.012]
　Dou Haoyu*,Zhu Yu,Zhou Wei..Effects of acute exposure to environmental endocrine disruptors on protein expression in rat hippocampus[J].Stroke and Nervous Diseases,2023,30(03):294-296,306.[doi:10.3969/j.issn.1007-0478.2023.03.012]
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环境内分泌干扰物急性暴露对大鼠海马组织蛋白表达水平的影响()
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《卒中与神经疾病》杂志[ISSN:1007-0478/CN:42-1402/R]

卷:
第30卷
期数:
2023年03期
页码:
294-296,306
栏目:
论著
出版日期:
2023-06-20

文章信息/Info

Title:
Effects of acute exposure to environmental endocrine disruptors on protein expression in rat hippocampus
文章编号:
1007-0478(2023)03-0294-04
作者:
窦浩瑜〓朱彧〓周为
300131 天津市双环邨街社区卫生服务中心全科（窦浩瑜）；天津市海河医院检验科（朱彧）；天津市人民医院神经内科[周为（通信作者）]
Author(s):
Dou Haoyu*;  Zhu Yu;  Zhou Wei.
*General Department, Tianjin Shuanghuancun Street Community Health Service Center, Tianjin 300131
关键词:
环境内分泌干扰物; 蛋白质组学; 氧化还原失衡
Keywords:
Environmental endocrine disruptors; Proteomics; Oxidation reduction imbalance
分类号:
R742
DOI:
10.3969/j.issn.1007-0478.2023.03.012
文献标志码:
A
摘要:
目的 通过蛋白组学技术考察环境内分泌干扰物（Environmental endocrine disruptors，EEDs）双酚A（Bisphenol A，BPA）和邻苯二甲酸二（2-乙基）乙酯\[Di-(2-ethylhcxyl)phthalate，DEHP\]急性暴露对大鼠海马组织蛋白表达水平的影响。 方法 选取健康清洁级初断乳雄性SD（Sprague dawley）品系大鼠20只，随机分为对照组和暴露组，每组各10只；对照组行生理盐水灌胃，暴露组大鼠经BPA 10 mg〖DK〗·kg-1〖DK〗·bw-1+DEHP 500 mg〖DK〗·kg-1〖DK〗·bw-1灌胃急性暴露4 h后取海马组织经高效液相色谱-串联质谱分析差异蛋白表达水平，通过GO（Gene ontology）分析考察生物过程（Biological process，BP）和细胞成分（Cell component，CC），京都基因与基因组百科全书（Kyoto encyclopedia of genes and genomes，KEGG）进行通路富集分析，通过生化法分析谷胱甘肽氧化还原系统蛋白水平的变化。 结果 经高效液相色谱-串联质谱分析共检测到蛋白4073个，其中与对照组比较，暴露组大鼠海马组织显著下调蛋白179个，显著上调蛋白230个；细胞因子分泌负调控、嘌呤核苷代谢过程、轴突引导的信号素-丛状蛋白信号通路等生物过程以及细胞器膜、线粒体、核膜等细胞成分发生显著改变；经KEGG分析发现谷胱甘肽代谢、突触功能、阿尔茨海默病等通路发生了显著改变，还原型谷胱甘肽（Glutathione，GSH）和谷胱甘肽还原酶（Glutathione reductase，GSR）水平显著降低，氧化型谷胱甘肽（Glutathione oxidized，GSSG）水平显著升高。 结论 BPA+DEHP急性暴露诱导大鼠海马组织蛋白表达水平改变，其机制可能与氧化还原失衡有关。
Abstract:
Objective To investigate the effects of environmental endocrine disruptors (EEDs) Bisphenol A (BPA) and Di-(2-ethylhcxyl phthalate) (DEHP) on protein expression in rat hippocampus. Methods 20 healthy and clean grade male Sprague dawley (SD) rats were selected and randomly divided into control group and exposure group, with 10 rats in each group. The control group received normal saline gavage, and the rats in the exposure group received BPA 10 mg/kg〖DK〗·bw+DEHP 500 mg/kg·bw gavage acutely for 4 hours. MS) analyze differential protein expression, and the biological process (BP) and cell component (CC) were analyzed by Gene Ontology (GO), pathway enrichment was analyzed through Kyoto Encyclopedia of Genes and Genomes (KEGG), the glutathione oxidation reduction system was analyzed by biochemistry assay. Results A total of 4073 proteins were detected by HPLC-MS/MS analysis. Compared with the control group, the hippocampus of the exposed group significantly down-regulated 179 proteins and significantly up-regulated 230 proteins. The BP of negative regulation of cytokine secretion, purine nucleoside metabolic process, semaphorin-plexin signaling pathway involved in axon guidance, the CC of organelle envelope, mitochondrion nuclear and envelope were significant changed. The KEGG analysis found that the pathways such as glutathione metabolism, synaptic function and Alzheimer’s disease were significant changed. The level of glutathione and glutathione reductase was significant decreased, the glutathione oxidized was increased. Conclusion BPA+DEHP exposure induces changes in protein expression in the hippocampus of rats, which may be related to functions such as purine metabolism, glutathione metabolism, synaptic function and Alzheimer’s disease.

参考文献/References:

[1] Rock KD,Gillera SEA,Devarasetty P,et al.Sex-specific behavioral effects following developmental exposure to tetrabromobisphenol A (TBBPA) in Wistar rats[J].Neurotoxicology,2019,75:136-147.
[2] Liang AA,Yin NY,Liang SX,et al.Bisphenol a and several derivatives exert neural toxicity in human neuron-like cells by decreasing neurite length[J].Food Chem Toxicol,2020,135:111015.
[3] Jocsak G,Ioja E,Kiss DS,et al.Endocrine disruptors induced distinct expression of thyroid and estrogen receptors in rat versus mouse primary cerebellar cell cultures[J].Brain Sci,2019,9(12):359.
[4] Mukherjee A,Su A,Rajan K.Deep learning model for identifying critical structural motifs in potential endocrine disruptors[J].J Chem Inf Model,2021,61(5):2187-2197.
[5] 穆轶,朱彧.抑肝散提取物对环境内分泌干扰物神经毒性的抑制作用[J].天津医科大学学报,2022,28(2):151-155.
[6] Jin XH,Wang JB,Zou S,et al.Cinobufagin triggers defects in spindle formation and Cap-Dependent translation in liver cancer cells by inhibiting the AURKA-mTOR-eIF4E axis[J].Am J Chin Med,2020,48(3):651-678.
[7] Elkfury JL,Antunes LC,Dal Moro Angoleri L,et al.Dysfunctional eating behavior in fibromyalgia and its association with serum biomarkers of brain plasticity (BDNF and S100B): an exploratory study[J].Arch Endocrinol Metab,2021,65(6):713-722.
[8] Setti SE,Reed MN.Network activity changes in the pathophysiology of Alzheimer’s disease: the role of aging and early entorhinal cortex dysfunction[J].Metab Brain Dis,2022,37(2):289-298.
[9] Taheri E,Riahi R,Rafiei N,et al.Bisphenol a exposure and abnormal glucose tolerance during pregnancy: systematic review and meta-analysis[J].Environ Sci Pollut Res Int,2021,28(44):62105-62115.〖ZK）〗
[10]〖JP2〗Santoro A,Scafuro M,Troisi J,et al.Multi-systemic alterations by chronic exposure to a low dose〖JP〗 of bisphenol a in drinking water: effects on inflammation and NAD+-Dependent deacetylase sirtuin1 in lactating and weaned rats[J].Int J Mol Sci,2021,22(18):9666.
[11]Ren J,Jiang FX,Wang ST,et al.Hydrophilic hindering and hydrophobic growing: a vesicle glycometabolism multi-drug combination therapeutic against Alzheimer’s disease[J].Biomater Sci,2021,9(19):6444-6460.
[12]Ruiz-Pérez G,Ruiz de Martín Esteban S,Marqués S,et al.Potentiation of amyloid beta phagocytosis and amelioration of synaptic dysfunction upon FAAH deletion in a mouse model of Alzheimer’s disease[J].J Neuroinflammation,2021,18(1):223.
[13]Torgasheva A,Malinovskaya L,Zadesenets KS,et al.Highly conservative pattern of sex chromosome synapsis and recombination in neognathae birds[J].Genes (Basel),2021,12(9):1358.
[14]Czapski GA,Babiec L,J〖KG-*5〗〖DD（*3〗′〖DD）〗〖KG-*3〗sko H,et al.Synaptic alterations in a transgenic model of tuberous sclerosis complex: relevance to autism spectrum disorders[J].Int J Mol Sci,2021,22(18):10058.

备注/Memo

备注/Memo:
基金项目：天津市卫生健康科技项目（KJ20084）
更新日期/Last Update: 2023-06-20