[1]张雪梅,邬合旭,袁雄.西格列汀调节Hippo/YAP信号通路对脑出血大鼠脑组织损伤的影响[J].卒中与神经疾病杂志,2025,32(01):46-50,69.[doi:10.3969/j.issn.1007-0478.2025.01.008]
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西格列汀调节Hippo/YAP信号通路对脑出血大鼠脑组织损伤的影响()
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《卒中与神经疾病》杂志[ISSN:1007-0478/CN:42-1402/R]

卷:
第32卷
期数:
2025年01期
页码:
46-50,69
栏目:
脑出血
出版日期:
2025-02-20

文章信息/Info

文章编号:
1007-0478(2025)01-0046-06
作者:
张雪梅邬合旭袁雄
066000 秦皇岛市中医医院病理科(张雪梅),脑外科(邬合旭 袁雄)
关键词:
西格列汀 蛋白激酶Hpo/Yes相关蛋白信号通路 脑出血 脑组织 损伤
分类号:
R743.34
DOI:
10.3969/j.issn.1007-0478.2025.01.008
文献标志码:
A
摘要:
目的 探究西格列汀(Sitagliptin,XGLT)调节蛋白激酶Hpo(Protein kinases,Hippo)/Yes相关蛋白(Yes-associated protein,YAP)对脑出血(Intracerebral hemorrhage,ICH)大鼠脑组织损伤的影响。方法 采用胶原酶Ⅶ脑部注射构建ICH模型,将构建成功的ICH大鼠随机分为ICH组、L-XGLT组(灌胃20 mg/kg XGLT)、H-XGLT组(灌胃50 mg/kg XGLT)、XMU组(灌胃50 mg/kg XGLT+腹腔注射0.7 mg/kg Hippo-YAP激动剂XMU-MP-1),ICH组、Sham组(只作切口,不注射胶原酶Ⅶ)灌胃等量的生理盐水,每组各6只; 对各组大鼠进行神经功能缺损评分、血脑屏障通透性检测; 醋酸铀和枸橼酸铅染色观察各组大鼠血脑屏障结构; 蛋白免疫印迹(Western blot,WB)检测Hippo,YAP,p-YAP(YAP的磷酸化蛋白)蛋白的表达水平; 免疫荧光检测YAP核易位; 酶联免疫吸附测定(Enzyme-linked immunosorbent assay,ELISA)检测白细胞介素(Interleukin,IL)-6、肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)、IL-1β的水平。结果 与Sham组比较,ICH组有明显水肿现象,血管内皮薄厚不均,细胞不紧密连接,且神经功能缺损评分、溴化乙锭(Ethidium bromide,EB)渗透率、Hippo,YAP蛋白表达及L-1β,IL-6,TNF-α水平升高,p-YAP蛋白表达水平降低(P<0.05); 与ICH组比较,L-XGLT组、H-XGLT组脑组织损伤改善、神经功能缺损评分、EB渗透率、Hippo,YAP蛋白表达及L-1β,IL-6,TNF-α水平降低,p-YAP蛋白表达水平升高(P<0.05),而XMU组逆转上述表现。结论 XGLT可能抑制Hippo/YAP信号通路来对ICH大鼠脑组织损伤产生影响。

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备注/Memo

备注/Memo:
基金项目:秦皇岛市科学技术研究与发展计划任务书(编号为202301A021)
更新日期/Last Update: 2025-02-20