[1]梁芬,陈强,郑安锐,等.栀子苷调节嗅球USF1来发挥抗抑郁作用[J].卒中与神经疾病杂志,2025,32(01):70-75.[doi:10.3969/j.issn.1007-0478.2025.01.012]
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栀子苷调节嗅球USF1来发挥抗抑郁作用()
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《卒中与神经疾病》杂志[ISSN:1007-0478/CN:42-1402/R]

卷:
第32卷
期数:
2025年01期
页码:
70-75
栏目:
论著
出版日期:
2025-02-20

文章信息/Info

文章编号:
1007-0478(2025)01-0070-06
作者:
梁芬陈强郑安锐滕小军顾文源江蓉
435000 黄石市中心医院(湖北理工学院附属医院)消化内科[梁芬 郑安锐 滕小军 顾文源 江蓉(通讯作者)]; 华中科技大学同济医学院附属梨园医院药剂科(陈强)
关键词:
栀子苷 慢性温和应激 嗅球 脂肪酸合酶 上游转录因子1
分类号:
R741.04
DOI:
10.3969/j.issn.1007-0478.2025.01.012
文献标志码:
A
摘要:
目的 研究慢性温和应激(Chronic mild stress,CMS)抑郁小鼠嗅球脂肪酸合酶(Fatty acid synthase, FASN)及其调节因子的表达水平变化,并初步探索栀子苷抗抑郁的分子机制。方法 将雄性 C57小鼠随机分为正常对照组、CMS组、栀子苷组和氟西汀组,构建CMS小鼠抑郁症模型; 造模成功后灌胃给予氟西汀(5 mg·kg -1·d -1)和栀子苷(50 mg·kg-1·d-1)进行干预(1次/d,持续2周),干预过程前期模型组小鼠继续给予CMS; 接着采用糖水偏好实验、旷场实验和强迫游泳实验评价小鼠抑郁样行为; 最后选用实时荧光定量聚合酶链反应(Real-time quantitative polymerasechain reaction,RT-qPCR)和 Western blot 检测嗅球脂肪酸合酶(Fatty acid synthase, FASN)、胆固醇调节元件结合蛋白1c(Sterol regulatory element-binding protein 1c,SREBP1c)、碳水化合物反应元件结合蛋白(Carbohydrate response element binding protein,ChREBP)、肝X受体α(Liver X receptor α,LXR-α)和上游转录因子1(Upstream transcription factor 1,USF1)的表达水平。结果 模型组小鼠出现抑郁样行为,嗅球 FASN 和 USF1 的mRNA 和蛋白表达水平显著降低(P<0.05),而SREBP1c,ChREBP和LXR-α的mRNA 表达水平无显著差异; 药物干预后CMS模型小鼠抑郁样行为显著缓解,嗅球 FASN 和 USF1 表达水平降低被逆转(P<0.05或P<0.01)。结论 嗅球 USF1/FASN 信号表达水平降低参与 CMS 模型小鼠抑郁发生,栀子苷能够逆转CMS 模型小鼠以上抑郁样行为,逆转 CMS 所致嗅球 USF1/FASN通路抑制。

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备注/Memo

备注/Memo:
基金项目:湖北省自然科学基金面上项目(2023AFB677)
更新日期/Last Update: 2025-02-20