[1]张茜,刘松燕,丁帅,等.母爱行为通过缓解神经炎症减少子代小鼠的焦虑行为[J].卒中与神经疾病杂志,2025,32(01):76-82,88.[doi:10.3969/j.issn.1007-0478.2025.01.013]
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母爱行为通过缓解神经炎症减少子代小鼠的焦虑行为()
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《卒中与神经疾病》杂志[ISSN:1007-0478/CN:42-1402/R]

卷:
第32卷
期数:
2025年01期
页码:
76-82,88
栏目:
论著
出版日期:
2025-02-20

文章信息/Info

文章编号:
1007-0478(2025)01-0076-08
作者:
张茜刘松燕丁帅石玉珂王志昊
430060 武汉大学人民医院神经内科和武汉大学人民医院神经退行性疾病研究中心[陈洪玉 王舰浩 李翔 高锋 余杭 王嘉贝 李芳 张茜 刘松燕 丁帅 石玉珂 王志昊(通信作者)]
关键词:
母爱行为 神经炎症 慢性不可预测轻度应激 焦虑行为 CCAAT增强子结合蛋白β
分类号:
R741.041
DOI:
10.3969/j.issn.1007-0478.2025.01.013
文献标志码:
A
摘要:
目的 研究母爱行为对子代小鼠成年后应激所致的焦虑行为的影响及分子机制。方法 首先,制作哺乳期母爱行为增加的小鼠模型,对其子代小鼠和对照(母爱行为未增加组)子代小鼠在成年后随机进行慢性不可预测应激(Chronic unpredictable mild stress, CUMS),未经历CUMS的小鼠作为对照,利用旷场实验和十字高架实验评估CUMS诱导的焦虑行为,酶联免疫吸附实验检测神经炎症相关因子水平; 然后,通过注射病毒过表达白介素-6(Interleukin-6,IL-6)后检测母爱增加的子代小鼠成年后CUMS所致焦虑行为的变化; 进一步的蛋白质免疫印迹、实时定量聚合酶链反应(Polymerase chain reaction,PCR)测定子代海马内炎症相关转录因子CCAAT增强子结合蛋白β(CCAAT-enhancer binding protein β,C/EBPβ)的水平及活性; 最后,观察C/EBPβ过表达是否阻断了小鼠母爱增加对成年子代焦虑行为的改善。结果 母爱行为可以抑制子代小鼠海马内C/EBPβ的活性,降低脑内IL-6水平,缓解神经炎症,改善成年后CUMS介导的焦虑行为。结论 母爱行为通过缓解子代小鼠脑内神经炎症,减少成年后CUMS介导的焦虑行为。

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金面上项目(82371439); 国家自然科学基金青年科学基金项目(82101479); 国家重点研究计划项目(2021YFA1302400); 湖北省实验动物研究领域项目(2022DFE021); 武汉大学人民医院交叉创新人才项目(JCRCZN-2022-002)
更新日期/Last Update: 2025-02-20