[1]陈伯勇 高庆春 王玉周 潘梦秋 叶锦龙 周丽华.MicroRNA-143-3p对缺糖缺氧的大鼠脑微血管内皮细胞的保护作用及机制[J].卒中与神经疾病杂志,2020,27(05):561-566.[doi:10.3969/j.issn.1007-0478.2020.05.001]
　Chen Boyong*,Gao Qingchun*,Wang Yuzhou,et al.Protective effect of miR-143-3p on brain microvascular endothelial cells with oxygen-glucose deprivation and its mechanism[J].Stroke and Nervous Diseases,2020,27(05):561-566.[doi:10.3969/j.issn.1007-0478.2020.05.001]
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MicroRNA-143-3p对缺糖缺氧的大鼠脑微血管内皮细胞的保护作用及机制()
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《卒中与神经疾病》杂志[ISSN:1007-0478/CN:42-1402/R]

卷:
第27卷
期数:
2020年05期
页码:
561-566
栏目:
论 著
出版日期:
2020-10-20

文章信息/Info

Title:
Protective effect of miR-143-3p on brain microvascular endothelial cells with oxygen-glucose deprivation and its mechanism
文章编号:
1007-0478(2020)05-0561-06
作者:
陈伯勇 高庆春 王玉周 潘梦秋 叶锦龙 周丽华
Author(s):
Chen Boyong*;  Gao Qingchun*;  Wang Yuzhou;  et al.
*Department of Neurology, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260
关键词:
脑卒中 缺糖缺氧 MicroRNA-143-3p 脑微血管内皮细胞
Keywords:
Stroke Oxygen-glucose deprivation MicroRNA-143-3p Brain microvascular endothelial cells
分类号:
R743.31
DOI:
10.3969/j.issn.1007-0478.2020.05.001
文献标志码:
A
摘要:
目的 探讨MicroRNA-143-3p(miR-143-3p)对缺糖缺氧的大鼠脑微血管内皮细胞的保护作用及机制。方法 建立大鼠脑微血管内皮细胞(rBMECs)缺糖缺氧(OGD)损伤模型,分为正常培养组和OGD组,qRT-PCR检测各组不同时间rBMECs中miR-143-3p的相对表达水平; 干扰rBMECs中miR-143-3p表达的实验将细胞分为正常培养组、OGD组、NC inhibitor+OGD组和miR-143-3p inhibitor+OGD组,qRT-PCR检测各组细胞中miR-143-3p相对表达水平; MTT、流式细胞术分别检测各组细胞的增殖、周期和凋亡情况。结果 与正常培养组比较,OGD组OGD处理2、4、6、8和10 h后rBMECs中miR-143-3p相对表达水平明显上调,且呈明显上升趋势; 转染miR-143-3p inhibitor能够显著降低OGD条件下rBMECs中miR-143-3p相对表达水平; 与正常培养组比较,OGD组细胞的增殖能力、周期转化能力显著降低,而细胞凋亡比例显著增加; 与NC inhibitor+OGD组比较,miR-143-3p inhibitor+OGD组细胞的增殖能力、周期转化能力显著增强,而细胞凋亡比例显著降低。结论 OGD处理显著抑制rBMECs的增殖、周期转化、促进凋亡; 干扰miR-143-3p表达对OGD条件下的rBMECs具有保护作用。
Abstract:
ObjectiveTo investigate the protective effect of microRNA-143-3p(miR-143-3p)on rat brain microvascular endothelial cells with oxygen-glucose deprivation(OGD)and its mechanism.Methods The rat brain microvascular endothelial cells(rBMECs)injury model induced by OGD was established. They were divided into normal culture group and OGD group, miR-143-3p expression levels in rBMECs of two groups at different time were detected by qRT-PCR. Interfering miR-143-3p expression in rBMECs, the experiment was divided into normal culture group, OGD group, NC inhibitor + OGD group and miR-143-3p inhibitor + OGD group. The relative expression levels of miR-143-3p in cells of four groups were detected by qRT-PCR. The cell proliferation, cycle and apoptosis in cells of four groups were assessed by MTT assay, flow cytometry assay, respectively.Results Compared with the normal culture group, the relative expression levels of miR-143-3p in rBMECs increased significantly after 2, 4, 6, 8 and 10 h after OGD treatment in OGD group, and showed a marked upward trend. Transfection of miR-143-3p inhibitor could significantly reduce miR-143-3p relative expression levels in rBMECs under OGD treatment. Compared with normal cultured group, the abilities of cell proliferation, cycle transformation in OGD group significantly decreased, while the apoptotic rate significantly increased. Compared with NC inhibitor + OGD group, the abilities of cell proliferation, cycle transformation in the miR-143-3p inhibitor + OGD group significantly increased, while the apoptotic rate significantly decreased.Conclusion OGD treatment significantly inhibited cell proliferation, cycle transformation of rBMECs, and promoted apoptosis. Interference miR-143-3p expression had protective effects on rBMECs under OGD conditions.

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备注/Memo

备注/Memo:
(2019-10-05收稿)作者单位:510260 广州医科大学附属第二医院神经内科[陈伯勇 高庆春(通信作者)]; 广东三九脑科医院神经内科(王玉周 潘梦秋 叶锦龙 周丽华)
更新日期/Last Update: 2020-10-20