[1]罗佳颖,牛璇,朱柯东,等.聚苯乙烯纳米塑料对大鼠主动脉平滑肌细胞泡沫化的影响[J].卒中与神经疾病杂志,2024,(01):3-7.[doi:10.3969/j.issn.1007-0478.2024.01.001]
　Luo Jiaying,Niu Xuan,Zhu Kedong,et al.Effects of Polystyrene Nanoplastics on Foam Cell Formation in Rat Aortic Smooth Muscle Cells[J].Stroke and Nervous Diseases,2024,(01):3-7.[doi:10.3969/j.issn.1007-0478.2024.01.001]
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聚苯乙烯纳米塑料对大鼠主动脉平滑肌细胞泡沫化的影响()
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《卒中与神经疾病》杂志[ISSN:1007-0478/CN:42-1402/R]

卷:
期数:
2024年01期
页码:
3-7
栏目:
论著
出版日期:
2024-02-20

文章信息/Info

Title:
Effects of Polystyrene Nanoplastics on Foam Cell Formation in Rat Aortic Smooth Muscle Cells
文章编号:
1007-0478(2024)01-0003-05
作者:
罗佳颖; 牛璇; 朱柯东; 文婷婷; 但亮; 张兆辉
430061 武汉大学人民医院神经内科\[罗佳颖 牛璇 朱柯东 文婷婷 但亮 张兆辉（通信作者）\]
Author(s):
Luo Jiaying;  Niu Xuan;  Zhu Kedong;  et al.
Department of Neurology, Renmin Hospital of Wuhan University, Wuhan 430060
关键词:
动脉粥样硬化; 血管平滑肌细胞; 泡沫细胞; 纳米塑料; 自噬
Keywords:
Atherosclerosis Vascular smooth muscle cells Nanoplastics Microplastics Autophagy
分类号:
R543.5
DOI:
10.3969/j.issn.1007-0478.2024.01.001
文献标志码:
A
摘要:
目的 探讨聚苯乙烯纳米塑料（Polystyrene nanoplastics，NPs）对大鼠主动脉平滑肌细胞（Rat aortic smooth muscle cells，RASMCs）泡沫化的影响。 方法 体外培养大鼠主动脉平滑肌细胞，用不同水平的直径为80纳米的绿色荧光NPs（80 nm NPs）处理RASMCs 24小时；采用细胞计数试剂盒（Cell counting kit-8，CCK8）检测细胞活力，并检测细胞释放的乳酸脱氢酶（Lactate dehydrogenase，LDH）活性以评估细胞损伤程度；利用DiI荧光标记的氧化型低密度脂蛋白（Dioctadecyl-3,3,3,3-tetramethylin docarbocyanine-oxidized low density lipoprotein,DiI-oxLDL）诱导平滑肌细胞泡沫细胞形成，并行油红O染色显示脂滴；采用蛋白免疫印迹法检测自噬相关指标（Microtubule-associated protein 1 light chain 3B，MAP1LC3B）的表达水平。 结果 80 nm NPs可被RASMCs吞噬并产生细胞毒性，呈水平依赖性促进RASMCs摄取oxLDL，自噬相关指标（LC3B）表达水平 降低。 结论 聚苯乙烯纳米塑料可被血管平滑肌细胞吞噬并促进泡沫细胞形成，其潜在机制可能是通过抑制RASMCs自噬来实现的。
Abstract:
Objective〖WTBZ〗 To investigate the role of polystyrene nanoplastics (NPs) in foam cell formation in rat aortic smooth muscle cells (RASMCs). 〖WTHZ〗Methods 〖WTBZ〗 RASMCs were treated with green fluorescent NPs of 80-nanometer diameter (80 nm NPs) at different concentrations for 24 hours. Cell viability was assessed using the CCK-8 assay. Lactate dehydrogenase (LDH) activity assay kit was used to measure LDH release as an indicator of cell damage. Foam cell formation was induced using DiI-labeled oxidized low-density lipoprotein (DiI-oxLDL). Oil Red O staining was performed to visualize lipid droplets. Protein immunoblotting was conducted to assess the expression levels of autophagy-related marker microtubule-associated protein 1 light chain 3B (MAP1LC3B/LC3B). 〖WTHZ〗Results 〖WTBZ〗 The results demonstrated that RASMCs internalized the 80 nm NPs, and their uptake was associated with concentration-dependent cytotoxicity. Furthermore, the NPs promoted the uptake of oxLDL by RASMCs and downregulated the expression level of the autophagy-related marker LC3B. 〖WTHZ〗Conclusion 〖WTBZ〗 The 80nm NPs promote the uptake of oxLDL by RASMCs and induce foam cell formation. The potential mechanism underlying this effect may involve the inhibition of autophagy in RASMCs.

参考文献/References:

[1] Libby P.The changing landscape of atherosclerosis[J].Nature,2021,592(7855):524-533.
[2] Lusis AJ.Atherosclerosis[J].Nature,2000,407(6801):233-241.
[3] Mr B,S S,Gk O.Vascular smooth muscle cells in atherosclerosis[J].Circulation research,2016,118(4):692-702.
[4] Andrady AL.Microplastics in the marine environment[J].Marine Pollution Bulletin,2011,62(8):1596-1605.
[5] Miranda D de A,de Carvalho-Souza GF.Are we eating plastic-ingesting fish?[J].Marine Pollution Bulletin,2016,103(1-2):109-114.
[6] Dong CD,Chen CW,Chen YC,et al.Polystyrene microplastic particles:in vitro pulmonary toxicity assessment[J].Journal of Hazardous Materials,2020,385:121575.
[7] Jin H.Evaluation of neurotoxicity in BALB/c mice following chronic exposure to polystyrene microplastics[J].Environmental Health Perspectives,2022,130(10):107002.
[8] Zhao T,Shen L,Ye X,et al.Prenatal and postnatal exposure to polystyrene microplastics induces testis developmental disorder and affects male fertility in mice[J].Journal of Hazardous Materials,2023,445:130544.
[9] Leslie HA,van Velzen MJM,Brandsma SH,et al.Discovery and quantification of plastic particle pollution in human blood[J].Environment International,2022,163:107199.〖ZK)〗
[10]Lu YY,Li H,Ren H,et al.Size-dependent effects of polystyrene nanoplastics on autophagy response in human umbilical vein endothelial cells[J].Journal of Hazardous Materials,2022,421:126770.
[11]Wang Y,Dubland JA,Allahverdian S,et al.Smooth muscle cells contribute the majority of foam cells in ApoE (Apolipoprotein E)-deficient mouse atherosclerosis[J].Arteriosclerosis,Thrombosis, and Vascular Biology,2019,39(5):876-887.
[12] Di Pietro N,Formoso G,Pandolfi A.Physiology and pathophysiology of oxLDL uptake by vascular wall cells in atherosclerosis[J].Vascular Pharmacology,2016,84:1-7.
[13]孙香莹,庄银,王玉邦,等.微塑料环境暴露与人体健康效应研究进展[J].环境科学研究,2023,36(5):1020-1031.
[14]Lee HS,Amarakoon D,Wei CI,et al. Adverse effect of polystyrene microplastics (PS-MPs) on tube formation and viability of human umbilical vein endothelial cells[J].Food Chem Toxicol,2021,154: 112356.
[15]Florance I,Ramasubbu S,Mukherjee A,et al.Polystyrene nanoplastics dysregulate lipid metabolism in murine macrophages in vitro[J].Toxicology,2021,458:152850.
[16]Florance I,Chandrasekaran N,Gopinath PM,et al.Exposure to polystyrene nanoplastics impairs lipid metabolism in human and murine macrophages in vitro[J].Ecotoxicology and Environmental Safety,2022,238:113612.
[17]Pi S,Mao L,Chen J,et al.The P2RY12 receptor promotes VSMC-derived foam cell formation by inhibiting autophagy in advanced atherosclerosis[J].Autophagy,2021,17(4):980-1000.

备注/Memo

备注/Memo:
基金项目：国家自然科学基金（82071183、82001245）
更新日期/Last Update: 2024-02-20