[1]金桐 叶樱泽 古丽娟 熊晓星.甲异靛对小鼠脑缺血再灌注损伤的保护作用及对CD68+细胞极化的影响[J].卒中与神经疾病杂志,2019,26(01):3-7，32.[doi:10.3969/j.issn.1007-0478.2019.01.001]
　Shi Xuesong..The protective effect of meisoindigo on cerebral ischemic/reperfusion injury and its influence on CD68+ cells polarization in mice[J].Stroke and Nervous Diseases,2019,26(01):3-7，32.[doi:10.3969/j.issn.1007-0478.2019.01.001]
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甲异靛对小鼠脑缺血再灌注损伤的保护作用及对CD68+细胞极化的影响()
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《卒中与神经疾病》杂志[ISSN:1007-0478/CN:42-1402/R]

卷:
第26卷
期数:
2019年01期
页码:
3-7，32
栏目:
论 著
出版日期:
2019-02-26

文章信息/Info

Title:
The protective effect of meisoindigo on cerebral ischemic/reperfusion injury and its influence on CD68+ cells polarization in mice
文章编号:
1007-0478(2019)01-0003-06
作者:
金桐 叶樱泽 古丽娟 熊晓星
430060 武汉大学人民医院神经外科[金桐 熊晓星(通信作者)],中心实验室(叶樱泽 古丽娟)
Author(s):
Shi Xuesong.
*Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan 430060
关键词:
甲异靛 脑缺血再灌注损伤 小胶质细胞/巨噬细胞极化
Keywords:
Meisoindigo Cerebral ischemic and reperfusion injury Microglia/macrophage polarization
DOI:
10.3969/j.issn.1007-0478.2019.01.001
文献标志码:
A
摘要:
目的 研究甲异靛在脑缺血再灌注损伤中的抗炎症反应及调节巨噬细胞/小胶质细胞极化的作用。方法 采用经颈内动脉线栓法造成大脑中动脉闭塞,闭塞60min后将栓线拔出以实现大脑中动脉血流再灌注,形成小鼠短暂性大脑中动脉阻塞(transient middle cerebral artery occlusion,tMCAO)模型,观察甲异靛对小鼠脑梗死范围、脑含水量、神经行为学的影响; 采用免疫荧光染色观察甲异靛对小鼠脑组织缺血区小胶质细胞/巨噬细胞极化的影响,Western blot法检测缺血区大脑皮层炎症信号通路相关分子TLR-4、p65/NF-κB以及炎症因子TNF-α和IL-1β的表达水平。结果 甲异靛可以减少脑缺血再灌注48 h后的脑梗死体积(P<0.01),改善神经功能评分(P<0.05),减轻脑水肿(P<0.01),降低TLR-4/NF-κB信号通路分子及炎症因子表达水平,减少脑梗死区小胶质细胞/巨噬细胞M0向M1极化(P<0.05),促进M0向M2极化(P<0.001)。结论 甲异靛可能通过改善脑水肿、调控小胶质细胞/巨噬细胞极化和抑制炎症反应,从而在脑缺血再灌注损伤中起保护作用。
Abstract:
ObjectiveTo explore the anti-inflammation effect of meisoindigo and its ability to modulate macrophage/microglia polarization in cerebral ischemia and reperfusion injury.Methods Transient focal ischemia was induced in C57BL/6J mice by middle cerebral artery occlusion(MCAO)for 60 min. Infarct volume, neurological scores and cerebral water content were evaluated 48 h after MCAO. TTC staining, immunofluorescence and western-blot analysis were used to assess the effects of meisoindigo on the cellular inflammatory response and macrophage/microglia polarization.Results Administration of meisoindigo reduced infarct size(P<0.01), improved neurological score(P<0.05), alleviated brain edema(P<0.01), decreased M0 to M1 polarization(P<0.05), prompted M0 to M2 polarization(P<0.001),and reduced inflammation in the brain 48 h after stroke.Conclusion Our results suggested that meisoindigo might alleviates cerebral ischemic injury by modulating macrophages/microglia polarization and alleviating inflammation in the infarct region, thus meisoindigo provided a potential therapeutic agent for treating ischemic stroke.

参考文献/References:


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备注/Memo

备注/Memo:
基金项目:国家自然科学基金面上项目(81571147)
作者单位:430060 武汉大学人民医院神经外科[金桐 熊晓星(通信作者)],中心实验室(叶樱泽 古丽娟)
更新日期/Last Update: 2019-02-20